Celigo Imaging Cytometer User Feedback

The Celigo is really multifunctional – it can do an awful lot. If you want to track growth rates, it will do it perfectly. If you want to do large analysis, like [embryoid bodies], it will be perfect.

The Celigo Imaging Cytometer is efficient and reliable and it has helped both expedite the research process as well as give me peace of mind of its reliability!

The Celigo is a great instrument for fluorescent tumor sphere counting and even fluorescent colony counting. I would recommend it! - Deepak Kanojia, Northwestern University

The Celigo is an interesting instrument to use. I enjoyed looking at the images to see immune cell killing. Working with our representative from Nexcelom was great! He was incredibly helpful and available at all times of the day!

The Celigo is capable of accurately counting cells and greatly assists in assays that involve cell death and migration.

I worked with our local Nexcelom representative to set up a Celigo seminar presentation/demo for my lab and some others from neighboring labs. He was very accommodating to our incredibly busy schedule and even brought us lunch, with plenty of dessert! The presentation was very informative and the Nexcelom team has been very present in the lab this week to demo and answer any questions!

We had the chance to have a quick mini demo with the Celigo Imaging Cytometer, with a full-length Celigo demo scheduled for the upcoming weeks. The Celigo was great, reasonably easy to use once the settings were dialed in and allowed us to visualize the cells and their action in ways not previously possible.

The Celigo Imaging Cytometer is very convenient and makes the work less labor-intensive. This instrument is very user-friendly and accurate!

The aspects of being able to image proliferation are key to our experiments. The Celigo Imaging Cytometer is very user friendly and the support staff is outstanding! - Pam Bogert, Mayo Clinic

We have three super heavy users for the Celigo who are becoming experts, and about four more using the Celigo on a weekly basis. So far we are all very impressed with the results! - UCLA Institute for Geno & Proteo

With the Celigo, we are performing proliferation experiments on cultured cells with ease, compared to previous methods. We can look at cyst growth and quantitate proliferation rates.

The optics on the Celigo are better than I expected, and the instrument is easy to calibrate!

I have been using the Celigo for 3D tumorsphere assays in 384 well plates and for 2D growth tracking in 6 well plates. The Celigo is relatively easy to use and the manual that comes with the software is extremely useful. The machine has allowed me to quickly analyze growth of cells in different conditions, both 2D and 3D, over time. The images taken by the instrument are crisp. Furthermore, the ability of the machine to accurately measure spheroid size and migration allows me to perform experiments in 384 well plates with ease!

The best part of working with Celigo is its ability to run analysis on the fly. Also, the assay-specific algorithms that come with the program are very useful, e.g. the wound healing analysis method with a well mask is the most convenient method I've used among multiple instruments and analysis software packages.

We love the Celigo as it is providing us many new ways to test and confirm our assays. It also provides us with live images of our cells post-sort and enables us to generate growth charts. - Mehrnoosh Abshari, NIH - NIDCR

The Celigo helps count many plates of adhesion cells in a quick time frame.

I liked the AOPI stain application and the rapid counting of the desired population. We are also interested in the Celigo. We just had a great Celigo seminar last week and we look forward to the in-lab demo this week! - Mahwish Natalia, Pfizer Inc.

Thanks to the Celigo, we are now performing and monitoring 3D tumor spheroid growth inhibition routinely and easily. The multiplexing capacities of the machine are used regularly for organelles visualization, apoptosis and cell cycle assays; which highly decreases our use of a standard flow cytometer and increases our throughput by using mostly 96 and 384 well plates. Overall, the Celigo is very user friendly and we are very happy with our acquisition.

One of the main cell lines for my project is pretty difficult to work with. I'm still in the early stages with the Celigo, but its confluence and apoptosis assays seem promising in helping move my project forward. It's really nice to not have to trypsinize my cells every time I want to perform an assay with them. - Caitlin Nichols, Dana-Farber Cancer Institute

It’s relatively simple to use – it’s selfexplaining actually, reliable, and the images you get out of it are really nice, underlining your results and you can make nice presentations with the Celigo images.

The Celigo is our "go to" for low resolution high speed scans. Particularly spheroid biology. I spoke highly of the system at the Cambridge HCA talk. - Steven Titus, NIH NCATS

The automation feature on the Celigo has greatly increased our work efficiency by integrating the imager to our robotic system. - Mandy Yim, Genentech

Every night we’re scanning between 50-100 plates. You can’t look at 50-100 plates by eye, so we just come in and review the scans and data each morning. The Celigo makes everyone more efficient.

We really utilize the high-throughput aspect of the Celigo. You get really nice statistics. You get a lot of data points. Instead of only having 3 data points you get 96 data points. You can look at small changes and actually get some statistical significance out of it.

The Celigo is really multifunctional – it can do an awful lot. If you want to track growth rates, it will do it perfectly. If you want to do large analysis, like [embryoid bodies], it will be perfect.

The Celigo has allowed our department to standardize and centralize the work within the institution.

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