Publications Referencing Celigo
Below is a list of peer reviwed journals, such as Nature, Breast Cancer Research and Journal of Cell Biology, that reference Celigo imaging cytometer.
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Author | Date | Title | Journal | Description of Cells | Description of how Celigo was used |
---|---|---|---|---|---|
Dominguez, Charli | November 2017 | Neutralization of IL-8 decreases tumor PMN-MDSCs and reduces mesenchymalization of claudin-low triple-negative breast cancer | JCI insight | MDA-MB-231, BT549, and Effector NK cells. | Celigo was used to determine the cell viability after staining with propidium iodide |
Kwang Ha, Tae | November 2017 | Baicalein Reduces Oxidative Stress in CHO Cell Cultures and Improves Recombinant Antibody Productivity | Biotechnology Journal | rCHO cells | Celigo was used to analyze ROS level of cells stained with CM-H2DCFDA. |
Xu, S | November 2017 | Basic Transcription Factor 3 Is Involved in Lung Cancer Growth and Progression Supplement: Supplement | Journal of Thoracic Oncology | Human NSCLC cells NCI-H1299 and A549 | Human NSCLC NCI-H1229 and A549 cells were transfected with BFTF3 shRNA lentiviral vector. Celigo was used to analyze cell proliferation, cell death, and viability of human NSCLC |
H, Liu | November 2017 | AK2 Is Overexpressed and Correlated with Increased Metastasis Potential and Tumorigenicity in Lung Adenocarcinoma: Supplement | Journal of Thoracic Oncology | LAD cells | Celigo cell imaging analyzer,
flow cytometric analysis, cell transwell assays, Immunofluorescence test and western blotting were performed to assess the effects of AK2 on proliferation, apoptosis, cell cycle, autophagy and epithelial-to-mesenchymal (EMT) phenotypes in LAD cells in vitro. |
Pan, Kung | November 2017 | ERH is up-regulated in bladder cancer and regulates the proliferation and apoptosis of T24 bladder cancer cells | Journal of Thoracic Oncology | bladder cancer cell lines T24 and 5637 | BrdU incorporation and
Celigo assays were used to examine the cell proliferation of ERHshRNA or scrambled-shRNA cell lines |
Dame, Keri | November 2017 | Derivation of Thyroid Progenitors from Embryonic Stem Cells through Transient, Developmental Stage-Specific Overexpression of Nkx2-1 | ProQuest Dissertations Publishing | Thyroid cells are derived from mouse embryonic stem cells | |
Filipovic, Nenad | November 2017 | Selenotriapine An isostere of the most studied thiosemicarbazone with pronounced pro-apoptotic activity, low toxicity and ability to challenge phenotype reprogramming of 3-D mammary adenocarcinoma tumors | Arabian Journal of Chemistry | MCF-7 tumor models | The Celigo was used to measure the area of the spheroids. Measurments were taken over an 8 day period and spheroid size was compared to size on day 0. Growth of treated and untreated spheroids were measured. |
Hanigan, Thomas | October 2017 | Scaffold dependent histone deacetylase (HDAC) inhibitor induced re-equilibration of the subcellular localization and posttranslational modification state of class I HDACs | Plos One | MCF-7 cells | MCF-7 cells were fixed with methanol and stained with PI. The Celigo was used to image the cells andperform cell cycle analysis. |
Quentin Li, Qingdi | October 2017 | Protein kinase D inhibitor CRT0066101 suppresses bladder cancer growth in vitro and xenografts via blockade of the cell cycle at G2/M | Cellular and Molecular Life Sciences | Bladder cancer cells: T24, T24T, TCCSUP, and UMUC1. Human uroepithelial cells: SV-HUC | Bladder cancer and human uroepithelial cells were incubated with a compound called CRT0066101. After an incubation period, the cells were stained with calcein AM, PI, and Hoechst. Celigo was used to capture images of the cells and determine the viability. |
Ortiz-Virumbrales, Maitane | October 2017 | CRISPR/Cas9-Correctable mutation-related molecular and physiological phenotypes in iPSC-derived Alzheimer’s PSEN2N141I neurons | Acta Neuropathologica Communications | 7889(s)B, 050643 (Control), 948 (AD1), 949(fControl), and 950 (AD2) iPSC lines | Celigo was used to capture images and analyze iPSC cell lines stained with PI. |
Hsu, Chih-Jung | October 2017 | Trans-acting oligodeoxythymidine phosphorothioate triester reagents for transient transfection optimized and facilitated by a high-throughput microbioreactor system | Biotechnology and Applied Biochemistry | HeLa cells | The Celigo was used to analyze the "the transfection efficiency of each dTtaPS-plasmid complex". Celigo captured images of GFP positive cells to determine the fluorescence intensity in each well. |
Zhuang, Peng-Yuan | September 2017 | Effect of TALEN-mediated IL-6 knockout on cell proliferation, apoptosis, invasion and anti-cancer therapy in hepatocellular carcinoma (HCC-LM3) cells |
Oncotarget | HCCLM3-wt or HCCLM3-IL6(-) cells | HCCLM3-wt or HCCLM3-IL6(-) were treated with sorafenib or IFN_. To determine the proliferation cells, cell counting kit-8 was added to the 96-well plates. Celigo was used to determine the absorbance of cells at 490 nm. |
Shaw, David | September 2017 | Development and Characterization of an Automated Imaging Workflow to Generate Clonally-Derived Cell Lines for Therapeutic Proteins | Biotechnology Progress | CHO-K1 Cells | The Celigo was used to count CHO-K1 cells stained with CellTracker Green CMFDA 0 days and 21 days after plating." Both CHO-K1 Green and CHO-K1. Red expressing CHO-K1 hosts were transfected with the same DNA preparation of mAb B" and plated into 384-well plates after electroporation. After the plates were imaged, the results were used to " drive automated hit-picking where 1,056 wells with growth were picked at random into 96-well plates." |
Tsuboi, Setskuko | September 2017 | Critical Review—Water-Soluble Near-Infrared Fluorophores Emitting over 1000 nm and Their Application to In Vivo Imaging in the Second Optical Window (1000–1400 nm) | ECS Journal of Solid State Science and Technology | HeLa cells | In order to determine the toxicity of NIR fluorophores, the Celigo was used to determine the number of HeLa cells 0-70 hours after treatment with NIR fluorophoes. |
Li, Wei | September 2017 | RDM1 gene Overexpression Represents a Therapeutic Target in Papillary Thyroid Carcinoma Running title: The new therapeutic target of papillary thyroid carcinoma | Endocrine Connections | K1 and TPC1 cells | K1 and TPC1 cells were stained post lentivirus transduction to determine the rate of siRNA gene knockdown. 96-well plates were scanned on the Celigo using bright field and the green fluorescent channel. After gating, the Celigo determined the total cell count and " the average integrated red fluorescence intensity". The Celigo was used to create a scatter plot of "green fluorescence area (in µm2) and integrated fluorescence intensity. |
Zhour, Yizhou | September 2017 | Beating the Odds: The Poisson Distribution of All Input Cells During Limiting Dilution Grossly Underestimates Whether a Cell Line is Clonally-Derived or Not | Biotechnology Progress | CHO-K1 cells | The Celigo was used to image plates initially and three weeks later to determine the distribution of "mAbX expressing CHO-K1 cells [that] were transfected with either mAbX-sp1 or mAbX-sp2 vectors". 384-well plates were imaged in bright field and fluorescent channels. |
Kessel, Sarah | September 2017 | Real-Time Apoptosis and Viability HighThroughput Screening of 3D Multicellular Tumor Spheroids Using the Celigo Image Cytometer | SLAS Discovery | MCTS produced from the glioblastoma cell line U87MG | The Celigo was used to examine the "the kinetic apoptotic and cytotoxic effects" of numerous compounds of multicellular tumor spheroids. The bright field channel, blue channel, red channel, green channel, and far red channel was used on the Celigo to image and analyze the plates. |
Miao, Ruoyu | September 2017 | Utility of the dual-specificity protein kinase TTK as a therapeutic target for intrahepatic spread of liver cancer. | Scientific Reports | Liver Cancer Cells | The Celigo was used to perform in situ cellular analysis on liver cancer cells after TTK inhibition. They also used Annexin V-FITC/PI Hoechst Apoptosis assay on the Celigo. They performed cell cycle analysis using BrdU and DAPI stains on the Celigo. Lastly, they used the Celigo to capture bright-field images of cell growth. |
Mi Gu, Sun | August 2017 | A synthetic cannabinoid JWH-210 reduces lymphoid organ weights and T-cell activator levels in mice via CB2 receptors | Naunyn-Schmiedeberg's Arch Pharmacol | Splenocytes and Thrymocytes | The Celigo was used to measure the cell viability of splenocytes after exposing cells to EthD-1 and calcein AM. It was also used to measure fluorescent intensity of splenocytes or thymocytes stained with DAPI and "secondary antibodies conjugated to AlexaFluor 488 and 594" |
Briffa, Romina | August 2017 | Unravelling the role of TRIB1 in colorectal cancer: a functional molecular pathology approach | EPMA Journal | three stable TRIB1 knock-down CRC cell line | The Celigo was used to monitor "cell cycle progression, proliferation, tumour growth, and invasion". |
Tomita, Kyoko | August 2017 | Mixed-lineage kinase 3 pharmacological inhibition attenuates murine nonalcoholic steatohepatitis | JCI insight | BMDM or THP-1 cells | The Celgio was used to determine the quantity of migrated BMDMs and THP-1 cells that were fluorescently labeled. |
He, Xuefeng | July 2017 | Opa interacting protein 5 acts as an oncogene in bladder cancer | Journal of Cancer Research Clinical Oncology | BC-T24 and BC-5637 | The Celigo was used to monitor cell growth of BC-T24 and BC-5637 after infection with shOIP5/shCtl. shOIP5 and shCtl fluorescence intensity was monitored using the Celigo and the cell counts were determined from the measured fluorescence intensity. This was to determine the "knockdown of shOIP5 suppressed cell growth". |
Leonidou, Andri | July 2017 | Identification and Validation of Driver Kinases from Next-Generation Sequencing Data | Kinase Signaling Networks | | |
Oliemuller, Erik | July 2017 | SOX11 promotes invasive growth and DCIS progression | The Journal of Pathology | MCF10A, MCF10DCIS.com, BT474, BT549, Cal148, HCC202, MX-1, and UACC893. | Cells were grown in a ULA plate to allow spheroids to form. The Celigo was used to take images of the spheroids after four days. After fourteen days, the Celigo was used to determine the mammosphere-forming efficiency by determining the quantity of mamospheres and dividing by the number of cells plated per well. The Celigo was also used to measure the size of the spheroids and measure the fluorescence of Caspase-3. |
Kessel, Sarah | June 2017 | TIReal-time viability and apoptosis kinetic detection method of 3D multicellular tumor spheroids using the Celigo Image CytometerTLE | Cytometry Part A. | multicellular tumor spheroids | The Celigo was used in combination with PI and caspase 3/7 stain to measure the rate of apoptosis and the viability of multicellular tumor spheroids |
Daubeuf, François | June 2017 | A Fast, Easy, and Customizable Eight-Color Flow Cytometric Method for Analysis of the Cellular Content of Bronchoalveolar Lavage Fluid in the Mouse | Current Protocols in Mouse Biology | BAL cells, T cells, B cells, neutrophils, eosinophils, and macrophages | |
Cherradi, S | June 2017 | Antibody targeting of claudin-1 as a potential colorectal cancer therapy | Journal of Experimental Medicine & Clinical Cancer Research | colorectal cancer cells | The Celigo was used to read 6-well plates that contained colorectal cancer cells using the single colony verification application. The tumorsphere application was also used and captured images of the tumorspheres. Also, the expression analysis assay was used to determine the fluorescent signals. During this experiment, they stained the cells with DAPI nuclear stain. Lastly, the Celigo was used to determine the apoptosis and growth rate of the tumorspheres. |
de Andrade Mello, Paola | June 2017 | Hyperthermia and associated changes in membrane fluidity potentiate P2X7 activation to promote tumor cell death | Oncotarget, Advance Publications | MCA38 colon cancer cells | The viability was determined after the cells were exposed to CCK-8 and the Celigo was used to capture images of the live cells. |
Singh, Veena | June 2017 | Performance of Biocept's sample collection for tumor cell analysis. | Tumor Biology | BT474 (HER2 amplified) and H3112 (ALK re-arranged) cells | The Celigo was used to count BT474 (HER2 amplified) or H3112 (ALK re-arranged) cells after being thawed. |
Nabbi, Arash | May 2017 | ING3 promotes prostate cancer growth by activating the androgen receptor | BMC Medicine | LNCaP, PC3, and DU145 cells | The Celgio was used to monitor cell proliferation of LNCaP, PC3, and DU145 cells in a 96-well plate. |
Kondo, Yasushi | May 2017 | Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells | Diabetologia | hiPSC lines: 648A1 and 692D2. Fibroblast-derived hiPSC lines: 409B2, 206B6 and 201B7. hESC lines: H9, KhES1 and KhES3. | Cells were immunostained with anti-insulin antibodies and the Celigo was used to determine the induction rate of INS+ cells. |
Huang, Ying | May 2017 | Identification of hMex-3A and its effect on human bladder cancer cell proliferation | Oncotarget | Bladder cancer cells lines:5637 and T24 | The Celigo was used to examine the proliferation after bladder cancer cells were transfected with an interference RNA sequence. |
Grav, Lise | May 2017 | Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells | Springer Link | CHO Cells | The Celigo was used to determine the cell survival and cell confluency. |
Ivanov, Delyan | May 2017 | High-Throughput Spheroid Screens Using Volume, Resazurin Reduction, and Acid Phosphatase Activity | Springer Link | References the Celigo as being capable of measuring spheroid size and volume in high throughput. | |
Chan, Leo | May 2017 | A high-throughput image cytometry-based screening method for the detection of IL2-induced peripheral blood mononuclear cell-mediated cytotoxicity | Cytotherapy | peripheral blood mononuclear cells (PBMC) | "Viability was determined by employing different staining techniques such as enzymatic stain, nucleic acid stain, multi-stain method, fluorescent stain, and trypan blue staining on the Celigo and Cellometer. " |
Chan, Leo | May 2017 | Assessment of Cell Viability with Single-, Dual-, and Multi-Staining Methods Using Image Cytometry | Springer Link | In this work, they "demonstrate a novel high-throughput cytotoxicity screening assay using the Celigo imaging-cytometry method". A 96 well plate was used with the Celigo to analyze the percent lysis of cells. | |
Cuny, Gregory | April 2017 | PYRIMIDINE COMPOUNDS AND METHODS USING THE SAME | United States Patent Application | SH-SY5Y-hTAU441 cell | The Celigo was used to assess the cytotoxicity of SH-SY5Y-hTAU441 cells. |
Gheller, Brandon | April 2017 | PYY regulates human skeletal muscle progenitor cell proliferation | The FASEB Journal | Skeletal Muscle stem cells (satelitte cells) | The Celigo was used to track percent confluence of cells over five days. |
Blum, Jamie | April 2017 | Short-term Inflammation Increases Proliferative Capacity of Human Skeletal Muscle Progenitor Cells from Young and Old Female Donors | The FASEB Journal | Muslce Progenitor Cells | The Celigo was used to track the percent confluence, number of nuclei, and percent cell death. |
Smith, Paul | April 2017 | Microenviroment Cytometry | Single Cell Analysis: Contemporary Research and Clinical Applications | multicellular tumor spheroids (MCTSs) | The Celigo was used to track the multicellular tumor spheroids health by staining with viability dye DRAQ7. They also looked at non-viable cells in the culture. |
Zhang, Haohai | April 2017 | Novel high-throughput cell-based hybridoma screening methodology using the Celigo Image Cytometer | Elsevier | Chinease hamster ovary (CHO) cells | "Demonstrate novel high-throughput screening methodology employing the Celigo Image Cytometer, which avoids nonspecific signals by contrasting antibody binding signals directly on living cells, with and without recombinant antigen expression." |
Venugopal, Jessica | April 2017 | Ouabain promotes partial epithelial to mesenchymal transition (EMT) changes in human autosomal dominant polycystic kidney disease (ADPKD) cells | Elsevier | kidney disease (ADPKD) cells | The Celigo was used in combination with an inverted microscope to monitor the wound healing of a cell monolayer. |
Yang, Mei-Lin | March 2017 | "IL-6 ameliorates acute lung injury in influenza virus infection" | Scientific Reports | fibroblasts isolated from IL-6 -/- mice and WT mice | Fibroblasts were isolated from IL-6-/- mice and WT mice. The Celigo was used to determine the cell count, doubling time, and apoptosis rate by immunofluorescence with anti-cleaved caspase-3 antibody. It was also used to monitor the number of virus infected cells engulfed by macrophages compared to IL-6/- macrophages. The macrophages were exposed to QD649 particles and stained with DAPI. |
Itoh, M | March 2017 | NanoCulture Plate: A scaffold‐based high‐throughput three‐dimensional cell culture system suitable for live imaging and co‐culture | Technology Platforms for 3D Cell Culture: A User's Guide | States how NCP can be used with the Celigo for measurments. | |
Slawny, Nicole | March 2017 | Physiologically relevant spheroid models for three‐dimensional cell culture | Technology Platforms for 3D Cell Culture: A User's Guide | Lists the Celigo as a type of automated 3D assay equipment | |
Quentin Li, Qingdi | March 2017 | Proteomic analysis of proteome and histone post-translational modifications in heat shock protein 90 inhibition-mediated bladder cancer therapeutics | Scientific Reports | 5637 bladder cancer cells | They wanted to determine the antitumor impact of HSP90 inhibitors in 5637 bladder cancer cells. They stained the cells and used the Celigo to examine the cell viability. |
Dall'Acqua, William | January 2017 | Enhanced tumor-targeting selectivity by modulating bispecific antibody binding affinity and format valence | Scientific Reports | NCI-H358 non-small cancer cells | Cell cytotoxicity studies were performed on a Celigo S Imaging cytometer. Overlay of brightfield with either green fluorescence or blue fluorescence identified and qualified CMFDA or BMQC positive cells using Celigo Image processing software. |
Li, Linfeng | January 2017 | 3D High-Content Screening of Organoids for Drug Discovery | Elsevier | None | This paper listed the Celigo as a 3D high content screening system |
Parker, Christopher | January 2017 | Ligand and Target Discovery byFragment-Based Screening in Human Cells | Elsevier | Human Cells | |
Cribbes, Scott | January 2017 | A Novel Multiparametric Drug-Scoring Method for High-Throughput Screening of 3D Multicellular Tumor Spheroids Using the Celigo Image Cytometer | SAGE Journals | glioblastoma cell line U87MG | In this work, we employed the Celigo Image Cytometer to examine the effects of 14 cancer drug compounds on 3D MCTS of the glioblastoma cell line U87MG in 384-well plates. |
Auberon, Florence | December 2016 | Isolation of novel stilbenoids from the roots of Cyrtopodium paniculatum (Orchidaceae) | Fitoterapia | human glioblastoma U-87MG cells | Celigo |
Braganza, Andrea | December 2016 | UBE3B is a calmodulin-regulated, mitochondria-associated E3 ubiquitin ligase | The Journal of Biological Chemistry | human cells | The cells were counted various times after plating. Colonies were also stained with Crystal violet dye and counted with the Celigo. |
Boss, Olivier | December 2016 | Methods and compositions for inducing differentiation of human brown adiopocyte progenitors | FPO | adipose tissue (BAT) progenitor cells | Cells were exposed to C1-BODIPY® 500/510 C12 (Molecular Probes #D-3823) and incubated for 3 to 6 hours. C1-BODIPY® 500/510 C12 is a fluorescent fatty acid analog that is sometimes utilized for lipid trafficking studies (ThermoFisher). Next, the cells were analyzed in bright field and fluorescence. They looked at the total fluorescent intensity per well. |
Wu, Yao | November 2016 | Cucurbitacin-I induces hypertrophy in H9c2 cardiomyoblasts through activation of autophagy via MEK/ERK1/2 signaling pathway | Toxicology Letters | H9c2 cells | The cell viabilities were detected by the Celigo Image Cytometer |
Kramer, Daniela | November 2016 | Strong antitumor synergy between DNA crosslinking and HSP90 inhibition causes massive premitotic DNA fragmentation in ovarian cancer cells | Cell Death & Differentiation | human ovarian cancer cell lines | |
Su, X | November 2016 | TAp63 suppresses mammary tumorigenesis through regulation of the Hippo pathway | Oncogene | mammary epithelial cells (MECs), mammary stem cells (MaSCs) and tumor-initiating cells (TICs) | |
Wu, Yao | November 2016 | Cucurbitacin-I induceshypertrophy in H9c2 cardiomyoblasts through activation of autophagy viaMEK/ERK1/2 signaling pathway | Toxicology Letters | H9c2 cells | The Celigo was used to analyze cell viability of H9c2 cells |
Cisneros Castillo, Liliana | October 2016 | A novel computer-assisted approach to evaluate multicellular tumor spheroid invasion assay | Scientific Reports | tumor and peripheral cells | To quantify the size of MCTS in invasion assays, the present of the Celigo Cytometer is an approach that comes already with a device that takes images automatically of an inserted plate. |
Kari, Vijayalakshmi | September 2016 | Loss of CHD1 causes DNA repair defects and enhances prostate cancer therapeutic responsiveness | The Embo Journal | Prostate cancer cells | |
Hamilton, Duane | September 2016 | Brachyury, a vaccine target, is overexpressed in triple- negative breast cancer | Society for Endocrinology | Triple-negative breast cancer cells | Nuclei were stained using DAPI and images were acquired using the Celigo S cell Imaging Cytometer |
Potapova, Tamara | August 2016 | Transcriptome analysis of tetraploid cells identifies cyclin D2 as a facilitator of adaptation to genome doubling in the presence of p53 | Molecular Biology of the Cell | Tetraplid cells | Multi-well plates with labeled cells were imaged on Celigo Imaging Cytometer. Images were quantified and each image typically contained several hundred of cells; 3-4 images were analyzed to determine the mean number of positive cells and the standard deviation |
Imamura, Yukio | August 2016 | Near-Infrared Emitting Pbs Quantum Dots for in Vivo Fluorescence imaging of the Thrombotic state in septic mouse brain | Molecules | HeLa cells | HeLa cells were plated at 6000 cells/well in 96 well plates. After culturing overnight, QD1100 was added to every well at a final concentration of 0-50 nM. The number of cells in each well was counted with the Celgio after 0,7,24,4,8, and 60 hours. |
Maguire, Sarah | August 2016 | 3D modelling identifies novel genetic dependencies associated with breast cancer progression in the isogenic MCF10 model | The Journal of Pathology | MCF10A cells | Comparison of pathway aberrations associated with progression identified that when cells are grown as 3D spheroids, they show perturbations of cancer-relevant pathways. The spheroids are was calculated using the Celigo S. |
Chan, Leo Li-Yang | August 2016 | A high-throughput AO/PI- based cell concentration and viability detection method using the Celigo image cytometry | Cytotechnology | Jurkat cells | The high-throughput AO/PI method described here allows for 96 well to 384-well plate samples to be analyzed in less than 7 mins, which greatly reduces the time required for the single sample based automated cell counter. |
Shan, Feng | July 2016 | Investigation of cancer drug penetration in 2D and 3D tumor cell culture models | University of Pittsburgh | Cal33 HNSCC cells | |
Bonasu, Surekha | July 2016 | Real-time Caspase 3/7 measurement of suspension and adherent cells using the Celigo Image cytometer | Poster | Jurkat cells | Monitoring by detecting a green caspase 3/7 signal but also perform an end point assay by counterstaining the cells with Hoechst and therby determined a percent nucleated cells that are apoptosis by imaging on the Nexcelom Celigo image cytometer. |
Chan, Leo Li-Yang | July 2016 | A high-throughput image cytometry-based screening method for the cytotoxic effect of drug compounds on 3D tumor spheroid | Cancer Research | Cancer cells | Five experiments were conducted demonstrating comparable results in 2D and 3D models using the image based high-throughput screening method for 3D tumor spheroids on 384- well ultra low attachment round bottom microplates using the Celigo image cytometer. |
Shirihai, Orian | July 2016 | Lysosome acidification by photoactivated nanoparticles restores autophagy under lipotoxicity | The Journal of Cell Biology | B- cells | Analysis parameters for images acquired by the Celigo were optimized to identify individual cells based on fluorescence. Cells were then fixed in 4% paraformaldehyde, stained with DAPI, and imaged using Celigo to count the total number of cells per well for normalization. |
Chan, Leo Li-Ying | June 2016 | High-throughput 3D tumor spheroid screening method for cancer drug discovery using Celigo Image Cytometry | Journal of Laboratory Automation | U87MG | Celigo was used to determine the seeding density for the tumor sphere formation of the cell line U87MG, and it was to measure the IC50 value, and the dose responses of 17-AGG, paclitaxel, TMZ and doxorubicin |
Ellegaard, Anne-Marie | June 2016 | Repurposing Cationic amphiphilic antihistamines for cancer treatment | EbioMedicine | Non-small cell lung cancer (NSCLC) | Celigo measured the cell death after 15 minutes of propidium iodide and Hoechst-33342 staining was employeed at 37 degrees celcius. |
Mazor, Yariv | June 2016 | Enhancement of Immune Effector Functions by modulating IgG's intrinsic affinity for target antigen | Immune cells, T-cells | Immune cells, T-cells | Target cells were stained with Calcein AM dye, and then seeded in a 96 well plate at a density of 1x10^4 cells/well in RPMI in 1640 with GlutaMAX with 5% FBS. Calcein AM positive cells were quantified by Celigo. |
Napoli, Marco | June 2016 | ΔNp63/DGCR8- Dependent MicroRNAs mediate therapeutic efficacy of HDAC inhibitors in Cancer | Cancer cell | Squamous cell carcinomas and lymphoma cells | To acess the efficacy of the compounds in affecting the protein levels of ΔNp63 and DGCR8, evaluating cells by immunofluoresence and quantified them with Celigo. |
Patterson, Lisa | May 2016 | In vitro assays for endothelial cell functions required for angiogenesis: Proliferation, motility, tubular differentiation, and matrix proteolysis | Angiogenesis Protocols | Umbilical vein endothelial cells, microvascular endthothelial cells | Lysosomal areas in the indicated fibroblasts treated for 12 hours with vehicle (V) or 19.5 or 39um C12-SM were measured with Celigo adherent cell cytometer after staining. |
Kildegaard, Helene Faustrup | May 2016 | Accelerated homology-directed targeted integration of transgenes in Chinese hamster ovary cells via CRISPR/Cas9 and fluorescent enrichment | Biotechnology & Bioengineering | CHO cells | Lysosomal areas in the indicated fibroblasts treated for 12 hours with vehicle (V) or 19.5 or 39um C12-SM were measured with Celigo adherent cell cytometer after staining. |
Tomokazu, Tanaka | April 2016 | Low-dose farnesyltransferase inhibitor suppresses HIF-1a and snail expression in Triple-negative breast cancer MDA-MB-231 cells in vitro | Journal of Cellular Physiology | MDA-MB-TNBC and MDA-MB-231 cells | Tumorspheres were directly counted by the Celigo Cell Cytometer at a 12-day culture. Pictures of each well were taken to conform spheres and aggregates of cells. |
Mattson, Emma | April 2016 | Understanding the role of RB-binding protein KDM5A in cancer cell proliferation | Illinois Mathematics and Science Academy | lung cancer cells | Focused on clarifying the role of KDM5A in a non-small cell lung cancer line under different concentrations of erlotinib, a common chemotherapy drug, by examinging the cell cycle and cell proliferation using the Celigo. |
Elisabeth, Corecelle-Termeau | April 2016 | Excess sphingomyelin disturbsATG9A trafficking and autophagosome closure | Autophagy | SMPD-1- deficient cells | Lysosomal areas in the indicated fibroblasts treated for 12 hours with vehicle (V) or 19.5 or 39um C12-SM were measured with Celigo adherent cell cytometer after staining. |
Amy, Wagers | April 2016 | Methods and compositions forrejuvenating skeletal muscle stem cells | FPO | Skeletal muscle stem cells | Wells containing myogenic colonies were either conunted by brightfield microscopy or fixed with 4% PFA and the number of cells per well was counted on a Celigo automated microscope as Hoechst-stained nuclei at the indicated time points |
Gayathri, Devi | April 2016 | Three-Dimensional culturesystems in cancer research: Focus on tumor spheroid model | Pharmacology & Therapeutics | Cancer stem cells | Cancer cells propagated in three-dimensional (3D) culture systems exhibit physiologically relevant cell-cell and cell-matrix interactions, gene expression and signaling pathway profiles, heterogeneity, and structural complexity that reflecdt in vivo tumors. |
Bidisha, Bhattacharya | April 2016 | Fine-tuning of macrophageactivation using synthetic rocaglate derivates | Scientific Reports | J7-21 cells | Celigo cytometer was used ot measure number of GFP-Ipr1 positive cells and GFP-Ipr1 fluorescence intensity per nucleus after counterstaining with nuclear stain DAPI. |
Peitzsch, Claudia | March 2016 | An epigenetic reprogramming strategy to re-sensitize radioresistant prostate cancer cells | Cancer Research | Cancer stem cells | After 14 days, cells were anaylzed and automatically scanned using the Celigo for 30 mins at 37 degrees celcius, washed with PBS at the end of the culture, then analyzed by the Celigo. |
Stanford, Elizabeth | March 2016 | The role of the aryl hydrocarbonreceptor in the development of the cells with the molecular and functionalcharacteristics of cancer stem- like cells | BMC Biology | Breast cancer stem cells | After cells were treated, harvested, dosed, and plated colonies were quantified with the Celigo after eight days. |
Mohammad, Tariq | January 2016 | Eukaryotic translationinitiation factor 5A (elF5A) is essential for HIF-1a activiation in hypoxia | Biochemical and Biophysical research communications | Tumor spheroids | Suppression of elF5A by siRNA oligomediated knockdown or treamtent with GC7, a deoxyhypusine synthase inhibitor, led to significant reduction of HIF-1a activity |
Edwin, Chang | November 2015 | AshwaMAX and withaferin Ainhbits gliomas in cellular and murine orthotopic models | Journal of Neuro-oncology | GBM2, GBM39 | Human parietal-cortical gliobastoma cells (GBM2, GBM39) were isolated from primary tumours while U87-MG was obtained commerically. |
Linfeng, Li | November 2015 | High-throughput imaging:Focusing in on drug discovery in 3D | Methods | MCT cells | 3D organotypic culture models such as organoids and multicellular tumor spheroids (MCTS) are becoming more widely used for drug discovery |
Henning, Gram Hensen | December 2015 | Versatile microscale screeningplatform for improving recombinant protein productivity in chinese hamsterovary cells | Scientific Reports | Ovarian cells | The platform consists of four techniques compatible with 96-well microplates:lipid-based transient transfection, cell cultivation in microplates, cell counting and antibody-independent product titer determination based on split-GFP complementation |
Sivapriya, Ponnurangam | December 2015 | Quinomycin A targets notchsignaling pathway in pancreatic cancer stem cells | Oncotarget | CSC cells | Quinomycin treatment resulted in significant inhibition of proliferation and colony formation in pancreatic cancer cell lines, but not in normal pancreatic epithelial cells |
Annika, Baude | December 2015 | Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination | Nucleic Acids research | HeLa cervic carcinoma cells | POGZ-depleted cells show a similar defect in DNA damage-induced RPA2 phosphorylation as HDGFRP2-depleted cells |
Andrew, Campbell | October 2015 | Mutation ofataxia-telangiectasia mutated is associated with dysfunctional glutathionehomeostasis in cerebellar astroglia | Glia | Mouse cells | Cerebellar atroglia isolated from Atm mutant mice show decreased expression of the cystine/glutamate exchanger subunit xCT, glutathione (GSH) reductase, and glutathione-S-transferase. |
Thilo, Riedl | October 2015 | High-throughput screening forinternalizing antibodies by homogeneous fluorescence imaging of apH-activated probe | Journal of Biomolecular Screening | A431, AU565, SKOV-3 cells | The pH-activated CypHer5E label becomes fluorescent upon internalization into the acidic environment of endocytic organelles, whereas background fluorescence of noninternalized CypHer5E is minimal. |
Narendran, Aru | September 2015 | Targeted inhibition of MEK1 bycobimetinib leads to differentiation and apoptosis in neuroblastoma cells | BioMed central | None | |
Simeonov, Anton | September 2015 | High- throughput fluorescence imaging approaches for drug discovery using in vitro and in vivo three-dimensional models | Expert Opinion Drug Discovery | None | |
Yoshida, Minoru | September 2015 | Identification of thedeterminants of 2-deoxyglucose sensitivity in cancer cells by shRNA libraryscreening | ScienceDirect | Cancer cells | |
Kaji, Keisuke | September 2015 | Reprogramming roadblocks are system dependent | ISSCR | (iPSC's) | |
Anant, Shrikant | August 2015 | RNA binding protein RBM3increases B-catenin signaling to increase stem cell characteristics incolorectal cancer cells | Wiley Online Library | Cancer stem cells | |
McGlennen, R | July 2015 | Comparison of Antimicrobial andwound healing agents on oral fibroblast viability and In-vivo bacterial load | Omics Online | Gingival fibroblast cells | |
Dobbelstein, Matthias | July 2015 | MicroRNA-101 suppresses tumorcell proliferation by acting as an endogenous proteasome inhibitor viatargeting the proteasome assembly factor POMP | ScienceDirect | Tumor cells | |
Dubrovska, Anna | June 2015 | Development of novelradiochemotherapy approaches targeting prostate tumor progenitor cells usingnanohybrids | International Journal of Cancer | Cancer stem cells | |
Gahl, William | June 2015 | Automated, High-throughputderivation, characterization and differentiation of induced pluripotent stemcells | Nature Methods | (iPSC's) | |
Mermod, Nicolas | June 2015 | Epigenetic regulatory elements:Recent advances in understanding their mode of action and use for recombinantprotein production in mammalian cells | Biotechnology Journal | mammalian cells | |
Lee, Dan | May 2015 | High- throughput direct cell counting- based natural killer cell mediated- cytotoxicity assay using Celigo Imaging Cytometry | The Journal of Immunology | NK cells | |
Sun, Yi | May 2015 | A reliable parameter tostandardize the scoring of stem cell spheres | PLOS one | Mouse embryonic stem cells | |
Jaattela, Marja | May 2015 | Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay | Autophagy | ||
Chang, Stephen | May 2015 | Rapid and efficient generationof transgene-free iPSC from a small volume of cryopreserved blood | Stem cell reviews and reports | hESC | online |
Kildegaard, Helene | April 2015 | One-step generation of tripleknockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment | Biotechnology Journal | CHO | Online |
Leach, M.O | March 2015 | Acquired resistance to EGFRtyrosine kinase inhibitors alters the metabolism of human head and necksquamous carcinoma cells and xenograft tumors | British Journal of Cancer | CAL/PJ HNSCC | online |
Nabergoj, Dominik, Elsa | December 2014 | Evaluation of anti-inflammatory and proapoptotic activities of synthetic clathrodin analogues in human THP-1 monocytic Leukemia cells | Thesis | THP-1 cells | online |
Flores, Elsa | November 2014 | Non-disruptively count and quantify fluorescence iPS colonies during 2 degree reprogramming: 7 min per 6 well plate, dual- fluorescence whole well imaging cytometry | Nature | Mouse embryonic stem cells(G4) | online |
Kolev, Vihren | November 2014 | P13K/mTOR dual inhibitor VS-5584preferentially target cancer stem cells | American Association for Cancer Research | Cancer stem cells | To detemine tumorsphere forming efficency, cells from the tissue culture were plated and enumerated using the Celigo. |
Karlin, Mondal | November 2014 | The oncogenic STP axis promotestriple-negative breast cancer via degradation of the REST tumor suppressor | Cell Reports | BT549 and MDA-MB231-LM2 breast cancer cells | Celigo was used to perform cell proliferation assays. |
Westbrook, Thomas | November 2014 | The oncogenic STP axis promotestriple-negative breast cancer via degradation of the REST tumor suppressor | Cell Reports | None | online |
Miura, Hiromi | October 2014 | Development of spheroid based high-throughput screening of cell-cell adhesion inhibitors to reverse acquired multicellular resistance | Cancer Research | A549 | online |
Randhawa, Zeng | October 2014 | Inhibition of large T antigenATPase activity as a potential strategy to develop anti-polyomavirus JC drugs | Antiviral Research | HEK293, COS7 | Cell counts were evaluated by Celigo to ascertain cytotoxicity of compounds. |
Glicksman, Marcie A. | October 2014 | Inhibition of large T antigenATPase activity as a potential strategy to develop anti-polyomavirus JC drugs | Antiviral Research | HEK293, COS7 | online |
Landmann, Proia | September 2014 | UDP glucuraonosyltransferase 1Aexpression levels determine the response of colorectal cancer cells to theheat shock protein 90 inhibitor ganetespib | Cell Death and Disease | human colorectal cancer cell lines | Cell confluence in drug treated and untreated wells was determined by Celigo with bright field. |
Fu, Creighton | September 2014 | Overcoming endocrine resistancedue to reduced PTEN levels in estrogen receptor-positive breast cancer byco-targeting mammalian target of rapamycin, protein kinase B, ormitogen-activated protein kinase kinase | Breast Cancer Research | MCF-7L, BT483, and T47D (human breast cancer) | Cell count was assessed on Celigo via methylene blue and bright field. |
Schiff, R | September 2014 | Overcoming endocrine resistancedue to reduced PTEN levels in estrogen receptor-positive breast cancer byco-targeting mammalian target of rapamycin, protein kinase B, ormitogen-activated protein kinase kinase | Breast Cancer Research | MCF7L (Human Breast Cancer), BT483, T47D | Online |
Xu, Farach-Carson | July 2014 | Three-dimensional in vitro tumormodels for cancer research and drug evaluation | Biotechnology Advances | tissue engineered-3D tumor models | Celigo measured spheroid diameter, permieter, and area which enabled assessments of motility, angiogenesis, and matrix invasion, as well as the actions of cell growth inhibitors. |
Brix, Rafn | July 2014 | Screening and identification ofsmall molecule inhibitors of ErbB2-induced invasion | Molecular Oncology | MCF-7 (human breast cancer) and SK-OV3 (human ovarian) | Cells were stained with Hoechst and PI and Celigo ascertained the total cell counts as well as number of dead cells. |
Jayanthan, Ruan | July 2014 | Aurora kinases as druggabletargets in pediatric leukemia: heterogeneity in target modulation activitiesand cytotoxicity by diverse novel therapeutic agents | PLOS One | pediatric and infant leukemia cell lines | Cell lines were stained with Alamar blue to assess cells survival via metabolic activity. |
Scheerlinck, Van Steendam | June 2014 | Detailed method description fornoninvasive monitoring of differentiation status of human embryonic stemcells | Analytical Biochemistry | human embryonic stem cells (hESCs) | Celigo evaluated colony confluence and measurement of stress level via Oct 4 expression. |
Pederson, Ronda | May 2014 | Accelerating genome editing inCHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool | Biotechnology and Bioengineering | CHO cells | Two-channel bright field and GFP images were captured by Celigo to count cells and determine transfection efficiency. |
Lund, Kildegaard | May 2014 | A versatile system for USERcloning-based assembly of expression vectors for mammalian cell engineering | PLOS One | CHO-S (Chinese hamster ovary clonal isolate) | Cells were plated in 6-well plates and transfected with GFP-tagged vectors before the addition of Hoechst stain. Two-channel (Hoechst and GFP) images were collected on the Celigo to determine cell count and transfection efficiency. |
Guo, Loh | May 2014 | Development of a magnetic 3Dspheroid platform with potential application for high-throughput drugscreening | Molecular Pharmaceutics | MCF-7 (human breast cancer) | Bright field images of the spheroids in 96-well plates were generated over a time course by Celigo. Growth kinetics of spheroid diameter, perimeter, and area were captured. |
Jaiswal, Lauritzen | May 2014 | S100A11 is required forefficient plasma membrane repair and survival of invasive cancer cells | Nature Communications | MCF-7 (human breast cancer) | Cell death was determined by Celigo using propidium idodide (PI) 96 hours after control or siRNA delivery. |
Yang, Chung | May 2014 | Systems analysis of the prostatetumor suppressor NKX3.1 supports roles in DNA repiar and luminal celldifferentiation | F1000 Research | LNCaP (human prostate cancer) | Cells were dual stained with Heochst and Alexa Fluor 594 and imaged by Celigo to ascertain cell proliferation. |
Guo, Loh | May 2014 | Development of a magnetic 3Dspheroid platform with potential application for high-throughput drugscreening | Molecular Pharmaceutics | multicellular tumor spheroids | Celigo captured bright field images over time to determine diameter, perimeter, and area of the spheroids with or without drug treatment. |
Kalineo, Thein | April 2014 | Acetaminophen and NAPQI aretoxic to auditory cells via oxidative and endoplasmic reticulumstress-dependent pathways | Hearing Research | HEI-OC1 cells (murine-derived auditory cells) | Celigo performed direct cell counts after treatment with control or drug over a time course. |
Spike, Kelber | April 2014 | CRIPTO/GRP78 signaling maintainsfetal and adult mammary stem cells ex vivo | Stem Cell Reports | MCF10A and fetal mammary stem cells | Celigo measured DNA content via Hoechst and cell proliferation through bright field images. |
Vissapragada, Contreras | March 2014 | Bidirectional crosstalk betweenperiventricular endothelial cells and neural progenitor cells promotes theformation of a neurovascular unit | Brain Research | Periventricular region endothelial cells (PVEC) and neural progenitor cells (NPC) | Cells were imaged by Celigo in 96-well Matrigel coated plates to ascertain the length and pixel intensity of 3D cords created by GFP-labeled ECs. |
Holembowski, Kramer | March 2014 | TAp73 is essential for germ celladhesion and maturation in testis | Journal of Cell Biology | Germ-Stertoli cell co-cultures | Celigo captured two-channel images (bright field and GFP) to quantify cells after lentiviral infection of adhesion genes. |
Emhemmed, Azouaou | March 2014 | Selective anticancer effects ofa synthetic flavagline on human Oct4-expressing cancer stem-like cells via ap38 MAPK-dependent caspase-3-dependent pathway | Biochemical Pharmacology | NT2/D1 (human embryonal carcinoma) | Celigo determined the rate of apoptosis via Annexin V/PI staining after flavagline derivative FL3 application. |
Trapnell, Cacchiarelli | March 2014 | The dynamics and regulators ofcell fate decisions are revealed by pseudotemporal ordering of single cells | Nature Biotechnology | human skeletal musucle myoblasts | Celigo performed whole well imaging with Hoechst and markers to determine the number of cells or nuclei, fraction of nueclei in MYH2- or CD13-positive cells, and total MYH2-positive area. |
Shih, Chung-Hsuan | February 2014 | Astroglial-Derived PeriostinPromotes Axonal Regeneration after Spinal Cord Injury | The Journal of Neuroscience | Rat Neurites | Neurite length was measured using Celigo cytometer to quantify the confluence of TujI-stained neurites, providing a quantitative measurement of neurite outgrowth. |
Fu, Morrison | February 2014 | Therapeutic potential of thedual EGFR/HER2 inhibitor AZD8931 in circumventing endocrine resistance | Breast Cancer Research Treatment | Tamoxifen resistant (TamRes) MCF-7 and T47D cells (both breast cancer) | Cells counts and rate of apoptosis via Annexin V-FITC staining was determined by Celigo. |
Schulz, Ramona | January 2014 | HER2/ErbB2 Activates HSF1 andThereby Controls HSP90 Clients Including MIF in HER2-Overexpressing BreastCancer | Cell Death and Disease | MDA-MB-453, and SK-BR-3 (Human Breast Cancer) | Cells were seeded and cultured for 1 day, then treated with CP724.714 (CP) for 24hr or left untreated and followed up to day 5. Cell confluence measured daily by Celigo. For cell survival, equal numbers of treated or untreated cells were plated into 12 well plates. With the Celigo cytometer, cell confluence was measured over indicated time periods w/Celigo |
Sproul, Andrew | January 2014 | Characterization and MolecularProfiling of PSEN1 Familial Alzheimers Disease iPSC Derived NeuralProgenitors | PLOS one | Fibroblast 11 and 11C, reprogramed to iPSCs | Immunostaining was performed. Hoechst was used to visualize DNA. The following antibodies were used: OCT4, SSea4, Nanog, Tra-160, Ki67, MAP2, Nestin, NeuNm TujI, NLRP2, NDP. Quantification of immunostaining was done on the Celigo 200-BFFL |
Giovannini, Marco | January 2014 | mTORC1 Inhibition Delays Growthof Neurofibromatosis Type 2 Schwannoma | Neuro-Oncology | Human Primary Schwann cells | Cells were fized in 4% paraformaldehyde and stained with anti-S100 protein,, followed by AI549-conjugated goat anti-rabbit secondary antibody and counterstained with Hoechst 33258. Cell surface areas were determined using the automated Celigo Cytometer. |
Postupalenko, Viktoriia | January 2014 | Intracellular Delivery ofFunctionally Active Proteins Using Self-AssemblingPyridulthiourea-polyehtlenimine | Journal of Controlled Release | U87 (Human Glioma), CaSki (Human small bowel mesentery), HeLa (Human Cervical Cancer) | Cells were seeded into 96-well plates at 10 4 cells/well the day before . Flow cytometry analysis was performed with a Celigo Cytometer after a 24 h incubation period on a suspension of freshly trypsinized cells |
Postupalenko, Sibler | January 2014 | Intracellular delivery offunctionally active proteins using self-assemblingpyridylthiourea-polyethylenimine | Journal of Controlled Release | U87 (human primary glioblastoma) | Cells were plated in 96-well plates and treated with GFP polyplexes. Celigo was used to determine intracellular eGFP delivery. |
Schulz, R. | January 2014 | HER2/ErbB2 Activates HSF1 andThereby Controls HSP90 Clients Including MIF in HER2-Overexpressing BreastCancer | Cell Death and Disease | ||
Ferree, Andrew | 2014 | Supplemental Material-characterization and molecular profiling of PSEN1 | Landes Bioscience | ||
Venkatanarayan, Raulji | 2014 | IAPP-driven metabolicreprogramming induces regression of p53-deficient tumours in vivo | Nature | H1299 (human lung adenocarcinoma) | Rates of apoptosis (via Annexin V/PI staining) and ROS generation (via CellRox Deep Red) were determined by Celigo. |
Chen, Hsing-Yu | March 2013 | Inhibition of red/Fyn/c-CBLPathway Function by Cdc42 Controls Tumour Initiation Capacity and TamoxifenSensitivity in Basal-like Breast Cancer Cells | EMBO Molecular Medicine | MDA-MB-231, MDA-MB-468, Hs578T, HCC1569, MCF-7m HCC38, HCC70, HCC1954, MCF-10A (Human Breast Cancer) | Cells were incubated with Calcein AM and PI for 15 mins, after which live and dead cells were counted utilizing the Celigo Cytometer. Levels of intracellular stress of oxidation were determined by CMH2DCFDA staining to the manufacturers instructions. Briefly cells were incubated with CM-H2DCFDA in the phenol red free medium in teh dark for 30 min, after which cells were washed once and fluorescent intensity of cells was determined using Celigo. |
Proschel, Christoph | December 2013 | Delayed Transplantation ofPrecursor Cell-Derived Astrocytes Provides Multiple Benefits in a Rat Modelof Parkinsons | EMBO Molecular Medicine | Cortical and dopaminergi mid brain neurons isolated from E18.5 Sprague-Dawley rats. | Cell survival was determined by co-labelling cultures with TujI and anti-tyrosine hydroxulase antibody. TujI+ or TujI+/TH+ neurons were counted using a Celigo Cytometer. |
Neradugomma, Naveen | December 2013 | Prolactin Signalling EnhancesColon Cancer Stemness by Modulating Notch Singaling in a Jak2-STAT3/ERKManner | Carcinogenesis | Colon cancer cell LinesHT29, HCT116, SW480, SW620, DLD1, and normal intenstinal epithelial fetal human colon (FHC) cells | Colosphere formation was assessed after 4-6 days, the number and size of colonospheres was determined using Celigo |
Xu, Cong | November 2013 | A Zebrafish Embryo CultureSystem Define Factors that Promote Vertebrate Myogenesis Across Species | Cell | Zebrafish cells | Zebra fish cells were iamged after 2 days using a Celigo cytometer for GFP, mCherry and BR signals. |
Neradugomma, N. | November 2013 | Prolactin induces Notchsignaling in a Jak2-STAT and Jak2-ERK pathway in colon cancer stem andprogenitor cells | Carcinogenesis | ||
Zangi, Lior | September 2013 | Modified mRNA Directs the Fateof Heart Progenitor Cells and Induces Vascular Regeneration After MyocardialInfarction | Nature Biotechnology | Skeletal muscle myotubes differentiated from primary mouse satellite cells | Cells were harvested and equal numbers were plated in each well of a 96-well plate in growth media. Cells were transfected with modified RNA after 3 d in differentiation media. Myotubes were transfected w/0, 0.3, 1 or 3 µg of GFP-modified RNA. Cells were fixed 16 h after transfection. Transfected myotubes were stained for skeletal muscle myosin heavy chain, 10 µg/ml Hoechst and rabbit anti-GFP Alexa-488. Pictures were taken from the whole well using the Celigo cytometer under blue, red and green channels. Cell viability was measured by quantifying the total number of nuclei in the transfected wells 16 h after transfection and normalizing them to the total number of nuclei in the non-transfected wells. |
Chen, Hsing-Yu | September 2013 | MEK1/2 Inhibition SuppressesTomixfen Toxicity on CNS Glial Progenitor Cells | The Journal of Neuroscience | O-2A/OPC (Astrocyte progentior/oligodendrocyte precursor cells), GRP (Glial-restricted precursor cells), astrocytes, neuroepithelial stem cells and oligodendrocytes | Cell number and death of O-2A/OPCs were analyzed by Calcein AM and PI Staining in live culture using a Celigo Cytometer. |
Ferree, Andrew | September 2013 | MitoTimer Probe Reveals theImpact of Autophagy, Fusion and Motility on Subcellular Distribution of Youngand Old Mitochondrial Protein and on Relative Mitochondrial Protein Age | Autophagy | MEF (Human Breast Cancer), COS (Monkey Kidney Tissue) | To quanity red and green fluorescence intensity per cell in MEF and COS cells, cells were imaged using Celigo. Analysis parameters for images acquired by Celigo were optimized to identify individual MEF and COS cell based on fluorescence and the avg. green and red fluorescnce intensity for each cell was determined at the different time points. |
Wang, Ying | August 2013 | Scalable Expansion of HumanInduced Pluripotent Stem Cells in the Defined Xeno-fress E8 Medium UnderAdherent and Suspension Culture Conditions | Journal of Stem Cell Research | BC1 (human primary effusion lymphoma ), TNC1 (Rat type 1 astrocytes) | The Total # of cell aggregates in a 1ml sample was analyzed by using the embryoid body analysis module in Celigo Imaging Cytometer. The avg. equivalent diameter of aggregates and the szie of each aggregate in the sample were also measured. |
He, Xianbao | August 2013 | The sst1 Resistance LocusRegulates Evasion of Type 1 Interferon Signalling by Chlamydia pneumoniae asa Disease Tolerance Mechanism | PLOS one: Pathogens | Bone Marrow Derived Macrophages | T-cell survival Assay: Cells were plated and treated with infected C. pneumoniae and at designated time points were stained with Hoechst and PI. Total cell number and dead cell number were counted using Celigo Cytometer |
Li, Jianqing | June 2013 | A Short Hairpin RNA Screen ofInterferon-Stimulated Genes Identifies a Novel Negative Regulator of theCellular Antiviral Response | Mbio. | Vero T144 (Kidney Epithelial Cells), NIH 3T3 (Human Fibroblasts), Hek293T (Human Embryonic Kideny cells), HeLa (Human Cervical Cancer) | HeLa cells in 96-well plates were transfected with individual ISGs tagged with 3X Flag. After 24h, cells were infected w/WNV for another 24h and then fixed, permeabilized, and costained for WNV envelope protein (MAbE18)and the nucleus using DAPI. Images were captured and processed using a Celigo cytometer (Cyntellect). Infected and uninfected populations were gated separately, and infectivity was measured as the % of infected cells from the total cell counts. |
Huang, Haiqing | June 2013 | iPSC-Derived B-Cells ModelDiabetes Due to Glucokinase Deficiency | Journal of Clincial Investigation | Patient Cell derived IPS to B-cell Model | Quantification of positively stained cells was performed using the celigo cytometer system. |
Bian, Shu | April 2013 | P2X7 Integrates PI2k/AKT andAMPK-PRAS40-mTOR Signaling Pathways to Mediate Tumor Cell Death | PLOS one | MC138 (Colon Cancer Cell Line), B16/F10 (Melanoma cell line) | Cells were seeded into Corning 3603 Black 96-well plates and grown for 24 hr before exposed to ATP for a short period of time. 16–24 hr later, cell growth was evaluated using the Celigo. BR images of live cells were captured using the Celigo Cell Counting application |
Escribano-Diaz, Cristina | March 2013 | A Cell Cycle Dependent RegulatoryCircuit Composed of 53BP1-RIF1 and BRCA1-CtlP Controls DNA Repair PathwayChoice | Molecular Cell | U2OS (human Osteosarcoma) | U2OS cells were transfected with siRNA in 96-well plates. At 48 hr post-transfection cells were treated w/neocarzinostatin for 15 min. After a 3 hr recovery, cells were extracted with 0.2% Triton X-100 in PBS for 10 min on ice, fixed with 4% paraformaldehyde (PFA), and processed for RPA32 immofluorescence. Nuclei were counterstained with DAPI. Cells were imaged using the Celigo laser scanning plate cytometer (Brooks Automation) and analyzed with the accompanying image analysis software. |
Vinci, Maria | January 2013 | Tumor Spheroid Based Migration Assays for Evaluation of Therapeutic Agents | Methods in Molecular Biology | ||
Lesovaya, Ekaterina | January 2013 | Combination of a SelectiveActivator of the Glucocorticoid Receptor Compound A with a Protease Inhibitoras a Novel Strategy for Chemotherapy of Hematologic Malignancies | Cell Cycle | CEM (T- cell acute lymphoblastoma leukemia), K562 ( chronic myeloblastic leukemia), NCEB (mantle cell lymphoma) and multiple MM.1R (glucocorticoid resistant) and MM1S (glucocorticoid sensitive) | The proliferation was measured by direct cell counting using Celigo Cytometer. Cells were plated at 10^4/well onto 24-well plates and cultured in complete medium in the presence of CdpA, Dex, Bortexomib or vehicle. |
Signh, Anjali | January 2013 | Profiliing Pathway SpecificNovel Therapeutics in Preclinical Assessment for CNS Atypical TeratoidRhabdoid Tumors | Molecular Oncology | BT12 and BT16 (CNS ATRT), KCCF1 (cerebral spinal fluid), Hs68 (Primary skin fibroblast), T98G [EGFR over-expressing glioblastoma multiform (GBM)] | ATRT cells were trypsinized and placed in 96-well plates @ a concentration of 5x10^3 cells per well. Increasing concentrations of study agents were added to these wells to a final volu,e of 200ul per well. After 4 days, cell survival was quantified by Celigo Cytometer |
Al-kasspooles, Mazin | January 2013 | Preclincal Antitumor Activity ofa Nanoparticulate SN38 | Journal of Investigative New Drugs | HT29, HCT116 (Human Colorectal Carcinoma) and H-meso-1 H226, H2052, MSTO-211H (Human Mesothelioma) | Cells were plated in 96-well black clear bottom plates. After overnight incubation, PI was added and live and dead cell numbers at time 0 were determined using the Celiigo cytometer. Drug treatments were then added to cells . Live and dead cell numbers were analyzed again 24, 48, and 72 after drug addition. |
Singh, Lun | January 2013 | Profiling pathway-specific noveltherapeutics in preclinical assessment for central nervous system atypicalteratoid rhabdoid tumors (CNS ATRT): Favorable activity of targeting EGFR-ErbB2 signaling with lapatinib | Molecular Oncology | atypical teratoid rhabdoid tumor (ATRT) cells | Cell survival in 96-well plates was determined by Celigo in bright field after 4 days treatment with drug or control. |
Stecklein, | August 2012 | SI Methods | PNAS | ||
Golipour, Azadeh | December 2012 | A Late Transition in SomaticCell Reprogramming Requires Regulators Distinct From the Pluripotency Network | Cell: Stem Cell | MEFs | Secondary MEFs as indicated in the schematics were stained for AO and DAPI and then scanned with a Celigo hgih-content microscope to quantigy AP-positive areas. Dox withdrawals was quantified for 4 SC and 4 SI clones using the Celigo system. In both screens, 3 days after transfection, cells were fixed and stained for Dppa24, counterstained with DAPI and then subjected to automated analysis using the Celigo system for quantifying colony formation |
Smith, Karen | September 2012 | Human Family with SequenceSimilarity 60 Member A (FAM60A) Protein: a New Subunit of the Sin3Deacetylase Complex | Molecular Cell Proteomic | A549, HepG2 (Human Liver Cancer) | A549 or HepG2 cells were transfected with control siRNAs or siRNAs against FAM60A. 3 days after transfection, cells were incubated for 5 hrs (A549) or 20hrs (HepG2) with BrdU. Cells were costained with DAPI. % incorporation of BrdU in each well was measured by fluorescence analysis using a Celigo Ceytometer |
Stecklein, Shane | August 2012 | BRCA1 amd HSP90 Cooperate inHomologous and Non-Homologous DNA Double Strand Break Repair and G2/MCheckpoint Activation | PNAS | HCC1937 (Human Breast Cancer) | DNA Synthesis, apoptosis and cell cycle distribution experiments in HCC1937 cells were performed on an LSRII flow cytometer or a Celigo adherent cell cytometer. |
Nabzdyk, Christoph | August 2012 | Differential Susceptibility ofHuman Primary Aortic and Coronary Artery Vascular Cells to RNA Interference | Journal of Biochemical and Biophysical Research Communications | Endothelial (EC) and vascular smooth muscle (SMC) cells | Cells were seeded at a density of 5000 cells/well of a 96-well plate. 24 hrs later cells were transfected with either non-targeting siRNA or non-targeting fluorescent red labelled siRNA using no transfection reagent., HiPerfect or Lipfectamine RNAiMax. Hoechst nuclei stain was used to label cells for counting. For data analysis an adherent cell cytometer Celigo was used. |
McKevitt, I. | April 2012 | SARS ORAL 2012 | British Journal Of Surgery Society Ltd. | ||
Borrego-Diaz, Emma | April 2012 | Overactivation of Ras SignalingPathway in CD133+ MPNST Cells | Journal of Neurooncology | MCF7L (Human Breast Cancer) | Functional analysis of cell growth and apoptosis was performed following knockdown of miRNAs using insitu cell cytometry (Celigo) |
Healy, N.A. | April 2012 | The Role of miRNAs in TamoxifenResistance in Breast Cancer | Cellular and Molecular Life Sciences | Human MPNST primary cells and mouse MPNSTs, S805, S462, T530, T532, SW10 (mouse Schwann cells) and HSCs | The spheres were counted after 40hr, 7 days and 10 days of incubation using both Celigo and a light microscope. |
Hsieh, Jeng-Long | March 2012 | Acquisition of an EnhancedAggressive Phenotypein Human Lung Cancer Cells Slected by Suboptimal Doses ofCisplatin Following Cell Deattachment and Reattachment | Journal of Cancer Letters | A549, H1299, H1299-RI~H1299-R5 (Human Lung Cancer Cell Lines) | To analyze cell proliferation 1000 cells were seeded in 96-well plates in the complete medium at 37C on day 0. The number of cells was counted daily from day 1 to day 4 using a Celigo cytometer according to themanufacturers instructions. The proliferation rate is expressed as a ratio of the numberof cells counted on days 2-4 by the number counted on day 1 |
Vinci, Maria | March 2012 | Advances in Establishment andAnalysis of 3D Tumor Spheroid-based Functional Assays for Target Validationand Drug Evaluation | BMC Biology | U-87 MG glioblastoma, SF188 glioblastoma, MDA-MB-231 (Human Breast Carcinoma),…37 other cancer cells lines. | For rapid, routine imaging and analysis of tumor spheroids, we utilized a Celigo cytometer, which is a bench top in situ celluar analysis system providing high quality, ful or partial images of wells using BR or FL illumination. |
Huang, Han-Hung | February 2012 | A Replacement for IsletEquivalents with Improved Reliability and Validity | Acta Diabetologica | Murine Pancreatic Islet cells | Isolated islet cells were iamged and counted with Celigo Cytometer |
Ponnurangam, Sivapriya | February 2012 | Honokiol in Combination withRadiation Targets Notch Signalling to Inhibit Colon Cancer Stem Cells | Molecular Cancer Therapeutics | HCT-116 (Human Colon Carcinoma), SW480 (Human Adenocarcinoma of the colon) | Colonosphere assay: The number and size of colonosphere were determined using Celigo |
Schulz, Ramona | January 2012 | Inhibiting the HSP90 ChaparoneDestabilizes Macrophage Migration Inhibitory Factor and Thereby InhibitsBreast Tumor Progression | Journal of Experimental Medicine | U2SO (Osteosarcoma), SW480 (Human adenocarcinoma of the colon) | Cell Confluence and cell numbers were evaluted over time by the Celigo Cytometer. Cell numbers (U2OS) or cell confluence (SW480) were measured using the Celigo cytometer using 49 squares per well |
Gall, Jonathan | January 2012 | Role of Mitofusin 2 in the RenalStress Response | PLOS one | Primary Murine Kidney Cells | Cells were fixed in 4% paraformaldehyde for 10 min, washed with PBS and stained with PBS containing Hoechst for 30 min before being imaged and counted by Celigo cytometer. |
Li, Dan | January 2012 | The Role of CYP3A4 mRNATranscript with Shortened 3'-Untranslated Region in HepatocyteDifferentiation, Liver Development and Repsonse to Drug Induction | Molecular Pharmacology | HepaRG Cells (Human Hepatic Cells) | The expression of RFP was analyzed in situ on an adherent cell cytometer to obtain cells images in both BR and red fuorescence, The ratios of cell densities from BR to red fluorescence in each entire well were analyzed by Celigo software. |
Yoshida, Shunsuke | November 2011 | Thrombospondin-2 Gene Silencingin Human Aortic Smooth Muscle Cells Improves Cell Attachment | Journal of the American College of Surgery | HAoSMC (Human Aortic Smooth Muscle Cell) | Scratch assay (wound healing attchment assay): cell positions were determined using BR and Confluence modes of Celigo, and cell counting of cells stained with Hoechst was performed |
Majumdar, Ishita | November 2011 | Synthetic Cyclohexenyl ChalconeNatural Products Possess Cytotoxic ActivitiesAgainst Prostate Cancer Cellsand Inhibit Cysteine Cathepsins in Vitro | Journal of Biochemical and Biophysical Research Communications | DU-145 and PC3 (Human Colon Cancer) Cells | Apoptotic cells stained with Hoechst were quantitatively analyzed with the Celigo cyotmeter |
Wang, Yen-Chao | November 2011 | Different Mechanisms forResistance to Trastuzumab Versus Lapatinib in HER2- Positive Breast Cancers,Role of Estrogen Receptor and HER2 Reactivation | Breast Cancer Research | BY474, UACC-812, AU-565, HCC-1569, HCC-1954, HCC-202, MDA-MB-361, MDA-MB-453, ZR75-30, SKBR-3, MCF7-HER2 (All Human Breast Cancer | Cells were incubated with Annexin V-FITC and DAPI for 30 min and analyzed by the Celigo Cytometer for Apoptosis. Cells were stained with EdU and the proliferation rate was analyzed by the Celigo cytometer |
Hoeksema, Kimberly | September 2011 | Systematic in-Vitro Evaluationof the NCI/NIH Developmental Therapeutics Program Approved Oncology Drug Setfor the Identification of a Candidate Drug Repertoire for MLL-RearrangedLeukemia | OncoTargets and Therapy | KOPN8 (B-Cell Precursor Leukemia), SEM (Human Acute Lymphoblastic Leukemia), B1 (Human B-cells), MOLT-3 (Human T-Lymphoblast), TIB-202 (acute monocytic leukemia) and Patient Bone Marrow Stromal (BMS) cells | Bright Field cell counting, viable cell numbers count and counting of primary leikemia samples. |
Las, Guy | August 2011 | Fatty Acids Suppress AutophagicTurnover in B-Cells | Journal of Biological Chemistry | INS1832/13 (Insulin-Producing ß-Cell Line) | Images of cells stained with Hoescht and PI, in paper. |
Wlodkowic, Donald | May 2011 | Wormomtry-on-a-Chip: InnovativeTechnologies for in Situ Analysis of Small Multicellular Organisms | Cytometry Part A. | None | Mentioned as an example of an image cytometer |
Lesovaya, E.A. | May 2011 | Antitumor Effect of Non-stroidGlucocorticoid Receptor Ligand CpdA on Leukemia Cell Lines CEM and K562 | Biochemistry (Moscow) | K562 (human myelogenous leukemia) cells, CEM (human lymphoblastic leukemia), transfected to create CEM-NF-_B-GFP-Luc, K562-NF-_B- GFP-Luc, CEM-AP1-GFP-Luc, and K562-AP1-GFP-Luc | Direct Count of suspension cells and a good cell count curve as an in paper figure |
Nabzdyk, Christoph | April 2011 | High-Throughput RNAi AssayOptimization Using Adherent Cell Cytometry | Journal of Translational Medicine | Human Aortic Smooth Muscle Cells | Cells were stained with Cell Tracker Green (Invitrogen) and with Hoechst nuclei stain. Plates were read using the adherent cell cytometer equipped with a brightfield and three fluorescent channels: a blue filter for the Hoechst, red filter for the siGLO Red, and green for the Cell Tracker Green cytoplasmic dye. |
Tingen, Candace | March 2011 | A Macrophage and ThecaCell-Enriched Stromal Cell Population Influences Growth and Survival ofImmature Murine Follicles in Vitro | Society for Reproduction and Fertility | Live, adhered ovarian stroma cells (Mouse) | Imaging and cell counts taken from cells stained with Hoechst and FITC |
Feng, Lili | March 2011 | Vascular CD39/ENTPD1 DirectlyPromotes Tumor Cell Growth by Scavenging Extracellular AdenosineTriphosphate | Neoplasia | U251 (Human Neuronal Glioblastoma), MDA-MB-231 (Human Breast Adenocarcinoma), A431 (Human Epidermal Carcinoma) and 5637 (Human Bladder Carcinoma) | Cell confluence was determined regularly for 16 days using the Celigo Cell Cytometer |
Bug, M | March 2011 | Anthracyclines induce theAcculumation of Mutant p53 Through E2F1-Dependant and Independent Mechanisms | Oncogene | Luciferase-expressing B16/F10, C57BL/6 (mouse melanoma) and Syngeneic C57BL/6murineMCA38 (colon cancer) | Cell growth of cells exposed to ATP was evaluated with images and cell counting using the Celigo counter. Images present in paper |
Kaestner, Phillip | February 2011 | Therapeutic Targeting of theMitotic Spindle Checkpoint Through Nanoparticle-Mediated siRNA DeliveryInhibits Tumor Growth in Vivo | Journal of Cancer Letters | HCT116 Cells (Human Colon Carcinoma) | Read cell numbers at 24hr increments |
Wlodkowic, Donald | July 2010 | Cytometry in Cell NecrobiologyRevisited. Recent Advances and New Vistas | Cytometry Part A. | None | Only mentioned as a comparable cytometric thenologu |
Sirmaci, Asli | May 2010 | A Truncating Mutation inSERPINB6 is Associated with Autosomal Recessive Nonsyndromic SensorineuralHearing Loss | The American Jounal of Human Genetics | HeLa cells, (Human Cervical Cancer) Transfected with SERPINB6-WT-GFP and SERPINB6-MUT-GFP | 3-Channel, 8-bit stitched images were generated covering whole wells to identify the surface area & # of cells along with fluorescent intensities. |
Hart, C.P. | May 2010 | Product Focus: High-ContentScreening and Imaging: Instrumentation, Analysis and Applications | Journal of Biomolecular Screening | None | Product focus, describeds product capabilities |