Publications Referencing Celigo

所有Celigo应用

Below is a list of peer reviwed journals, such as Nature, Breast Cancer Research and Journal of Cell Biology, that reference Celigo imaging cytometer.

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Author	Date	Title	Journal	Description of Cells	Description of how Celigo was used
Dominguez, Charli	November 2017	Neutralization of IL-8 decreases tumor PMN-MDSCs and reduces mesenchymalization of claudin-low triple-negative breast cancer	JCI insight	MDA-MB-231, BT549, and Effector NK cells.	Celigo was used to determine the cell viability after staining with propidium iodide
Kwang Ha, Tae	November 2017	Baicalein Reduces Oxidative Stress in CHO Cell Cultures and Improves Recombinant Antibody Productivity	Biotechnology Journal	rCHO cells	Celigo was used to analyze ROS level of cells stained with CM-H2DCFDA.
Xu, S	November 2017	Basic Transcription Factor 3 Is Involved in Lung Cancer Growth and Progression Supplement: Supplement	Journal of Thoracic Oncology	Human NSCLC cells NCI-H1299 and A549	Human NSCLC NCI-H1229 and A549 cells were transfected with BFTF3 shRNA lentiviral vector. Celigo was used to analyze cell proliferation, cell death, and viability of human NSCLC
H, Liu	November 2017	AK2 Is Overexpressed and Correlated with Increased Metastasis Potential and Tumorigenicity in Lung Adenocarcinoma: Supplement	Journal of Thoracic Oncology	LAD cells	Celigo cell imaging analyzer, flow cytometric analysis, cell transwell assays, Immunofluorescence test and western blotting were performed to assess the effects of AK2 on proliferation, apoptosis, cell cycle, autophagy and epithelial-to-mesenchymal (EMT) phenotypes in LAD cells in vitro.
Pan, Kung	November 2017	ERH is up-regulated in bladder cancer and regulates the proliferation and apoptosis of T24 bladder cancer cells	Journal of Thoracic Oncology	bladder cancer cell lines T24 and 5637	BrdU incorporation and Celigo assays were used to examine the cell proliferation of ERHshRNA or scrambled-shRNA cell lines
Dame, Keri	November 2017	Derivation of Thyroid Progenitors from Embryonic Stem Cells through Transient, Developmental Stage-Specific Overexpression of Nkx2-1	ProQuest Dissertations Publishing	Thyroid cells are derived from mouse embryonic stem cells
Filipovic, Nenad	November 2017	Selenotriapine An isostere of the most studied thiosemicarbazone with pronounced pro-apoptotic activity, low toxicity and ability to challenge phenotype reprogramming of 3-D mammary adenocarcinoma tumors	Arabian Journal of Chemistry	MCF-7 tumor models	The Celigo was used to measure the area of the spheroids. Measurments were taken over an 8 day period and spheroid size was compared to size on day 0. Growth of treated and untreated spheroids were measured.
Hanigan, Thomas	October 2017	Scaffold dependent histone deacetylase (HDAC) inhibitor induced re-equilibration of the subcellular localization and posttranslational modification state of class I HDACs	Plos One	MCF-7 cells	MCF-7 cells were fixed with methanol and stained with PI. The Celigo was used to image the cells andperform cell cycle analysis.
Quentin Li, Qingdi	October 2017	Protein kinase D inhibitor CRT0066101 suppresses bladder cancer growth in vitro and xenografts via blockade of the cell cycle at G2/M	Cellular and Molecular Life Sciences	Bladder cancer cells: T24, T24T, TCCSUP, and UMUC1. Human uroepithelial cells: SV-HUC	Bladder cancer and human uroepithelial cells were incubated with a compound called CRT0066101. After an incubation period, the cells were stained with calcein AM, PI, and Hoechst. Celigo was used to capture images of the cells and determine the viability.
Ortiz-Virumbrales, Maitane	October 2017	CRISPR/Cas9-Correctable mutation-related molecular and physiological phenotypes in iPSC-derived Alzheimer’s PSEN2N141I neurons	Acta Neuropathologica Communications	7889(s)B, 050643 (Control), 948 (AD1), 949(fControl), and 950 (AD2) iPSC lines	Celigo was used to capture images and analyze iPSC cell lines stained with PI.
Hsu, Chih-Jung	October 2017	Trans-acting oligodeoxythymidine phosphorothioate triester reagents for transient transfection optimized and facilitated by a high-throughput microbioreactor system	Biotechnology and Applied Biochemistry	HeLa cells	The Celigo was used to analyze the "the transfection efficiency of each dTtaPS-plasmid complex". Celigo captured images of GFP positive cells to determine the fluorescence intensity in each well.
Zhuang, Peng-Yuan	September 2017	Effect of TALEN-mediated IL-6 knockout on cell proliferation, apoptosis, invasion and anti-cancer therapy in hepatocellular carcinoma (HCC-LM3) cells	Oncotarget	HCCLM3-wt or HCCLM3-IL6(-) cells	HCCLM3-wt or HCCLM3-IL6(-) were treated with sorafenib or IFN_. To determine the proliferation cells, cell counting kit-8 was added to the 96-well plates. Celigo was used to determine the absorbance of cells at 490 nm.
Shaw, David	September 2017	Development and Characterization of an Automated Imaging Workflow to Generate Clonally-Derived Cell Lines for Therapeutic Proteins	Biotechnology Progress	CHO-K1 Cells	The Celigo was used to count CHO-K1 cells stained with CellTracker Green CMFDA 0 days and 21 days after plating." Both CHO-K1 Green and CHO-K1. Red expressing CHO-K1 hosts were transfected with the same DNA preparation of mAb B" and plated into 384-well plates after electroporation. After the plates were imaged, the results were used to " drive automated hit-picking where 1,056 wells with growth were picked at random into 96-well plates."
Tsuboi, Setskuko	September 2017	Critical Review—Water-Soluble Near-Infrared Fluorophores Emitting over 1000 nm and Their Application to In Vivo Imaging in the Second Optical Window (1000–1400 nm)	ECS Journal of Solid State Science and Technology	HeLa cells	In order to determine the toxicity of NIR fluorophores, the Celigo was used to determine the number of HeLa cells 0-70 hours after treatment with NIR fluorophoes.
Li, Wei	September 2017	RDM1 gene Overexpression Represents a Therapeutic Target in Papillary Thyroid Carcinoma Running title: The new therapeutic target of papillary thyroid carcinoma	Endocrine Connections	K1 and TPC1 cells	K1 and TPC1 cells were stained post lentivirus transduction to determine the rate of siRNA gene knockdown. 96-well plates were scanned on the Celigo using bright field and the green fluorescent channel. After gating, the Celigo determined the total cell count and " the average integrated red fluorescence intensity". The Celigo was used to create a scatter plot of "green fluorescence area (in µm2) and integrated fluorescence intensity.
Zhour, Yizhou	September 2017	Beating the Odds: The Poisson Distribution of All Input Cells During Limiting Dilution Grossly Underestimates Whether a Cell Line is Clonally-Derived or Not	Biotechnology Progress	CHO-K1 cells	The Celigo was used to image plates initially and three weeks later to determine the distribution of "mAbX expressing CHO-K1 cells [that] were transfected with either mAbX-sp1 or mAbX-sp2 vectors". 384-well plates were imaged in bright field and fluorescent channels.
Kessel, Sarah	September 2017	Real-Time Apoptosis and Viability HighThroughput Screening of 3D Multicellular Tumor Spheroids Using the Celigo Image Cytometer	SLAS Discovery	MCTS produced from the glioblastoma cell line U87MG	The Celigo was used to examine the "the kinetic apoptotic and cytotoxic effects" of numerous compounds of multicellular tumor spheroids. The bright field channel, blue channel, red channel, green channel, and far red channel was used on the Celigo to image and analyze the plates.
Miao, Ruoyu	September 2017	Utility of the dual-specificity protein kinase TTK as a therapeutic target for intrahepatic spread of liver cancer.	Scientific Reports	Liver Cancer Cells	The Celigo was used to perform in situ cellular analysis on liver cancer cells after TTK inhibition. They also used Annexin V-FITC/PI Hoechst Apoptosis assay on the Celigo. They performed cell cycle analysis using BrdU and DAPI stains on the Celigo. Lastly, they used the Celigo to capture bright-field images of cell growth.
Mi Gu, Sun	August 2017	A synthetic cannabinoid JWH-210 reduces lymphoid organ weights and T-cell activator levels in mice via CB2 receptors	Naunyn-Schmiedeberg's Arch Pharmacol	Splenocytes and Thrymocytes	The Celigo was used to measure the cell viability of splenocytes after exposing cells to EthD-1 and calcein AM. It was also used to measure fluorescent intensity of splenocytes or thymocytes stained with DAPI and "secondary antibodies conjugated to AlexaFluor 488 and 594"
Briffa, Romina	August 2017	Unravelling the role of TRIB1 in colorectal cancer: a functional molecular pathology approach	EPMA Journal	three stable TRIB1 knock-down CRC cell line	The Celigo was used to monitor "cell cycle progression, proliferation, tumour growth, and invasion".
Tomita, Kyoko	August 2017	Mixed-lineage kinase 3 pharmacological inhibition attenuates murine nonalcoholic steatohepatitis	JCI insight	BMDM or THP-1 cells	The Celgio was used to determine the quantity of migrated BMDMs and THP-1 cells that were fluorescently labeled.
He, Xuefeng	July 2017	Opa interacting protein 5 acts as an oncogene in bladder cancer	Journal of Cancer Research Clinical Oncology	BC-T24 and BC-5637	The Celigo was used to monitor cell growth of BC-T24 and BC-5637 after infection with shOIP5/shCtl. shOIP5 and shCtl fluorescence intensity was monitored using the Celigo and the cell counts were determined from the measured fluorescence intensity. This was to determine the "knockdown of shOIP5 suppressed cell growth".
Leonidou, Andri	July 2017	Identification and Validation of Driver Kinases from Next-Generation Sequencing Data	Kinase Signaling Networks
Oliemuller, Erik	July 2017	SOX11 promotes invasive growth and DCIS progression	The Journal of Pathology	MCF10A, MCF10DCIS.com, BT474, BT549, Cal148, HCC202, MX-1, and UACC893.	Cells were grown in a ULA plate to allow spheroids to form. The Celigo was used to take images of the spheroids after four days. After fourteen days, the Celigo was used to determine the mammosphere-forming efficiency by determining the quantity of mamospheres and dividing by the number of cells plated per well. The Celigo was also used to measure the size of the spheroids and measure the fluorescence of Caspase-3.
Kessel, Sarah	June 2017	TIReal-time viability and apoptosis kinetic detection method of 3D multicellular tumor spheroids using the Celigo Image CytometerTLE	Cytometry Part A.	multicellular tumor spheroids	The Celigo was used in combination with PI and caspase 3/7 stain to measure the rate of apoptosis and the viability of multicellular tumor spheroids
Daubeuf, François	June 2017	A Fast, Easy, and Customizable Eight-Color Flow Cytometric Method for Analysis of the Cellular Content of Bronchoalveolar Lavage Fluid in the Mouse	Current Protocols in Mouse Biology	BAL cells, T cells, B cells, neutrophils, eosinophils, and macrophages
Cherradi, S	June 2017	Antibody targeting of claudin-1 as a potential colorectal cancer therapy	Journal of Experimental Medicine & Clinical Cancer Research	colorectal cancer cells	The Celigo was used to read 6-well plates that contained colorectal cancer cells using the single colony verification application. The tumorsphere application was also used and captured images of the tumorspheres. Also, the expression analysis assay was used to determine the fluorescent signals. During this experiment, they stained the cells with DAPI nuclear stain. Lastly, the Celigo was used to determine the apoptosis and growth rate of the tumorspheres.
de Andrade Mello, Paola	June 2017	Hyperthermia and associated changes in membrane fluidity potentiate P2X7 activation to promote tumor cell death	Oncotarget, Advance Publications	MCA38 colon cancer cells	The viability was determined after the cells were exposed to CCK-8 and the Celigo was used to capture images of the live cells.
Singh, Veena	June 2017	Performance of Biocept's sample collection for tumor cell analysis.	Tumor Biology	BT474 (HER2 amplified) and H3112 (ALK re-arranged) cells	The Celigo was used to count BT474 (HER2 amplified) or H3112 (ALK re-arranged) cells after being thawed.
Nabbi, Arash	May 2017	ING3 promotes prostate cancer growth by activating the androgen receptor	BMC Medicine	LNCaP, PC3, and DU145 cells	The Celgio was used to monitor cell proliferation of LNCaP, PC3, and DU145 cells in a 96-well plate.
Kondo, Yasushi	May 2017	Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells	Diabetologia	hiPSC lines: 648A1 and 692D2. Fibroblast-derived hiPSC lines: 409B2, 206B6 and 201B7. hESC lines: H9, KhES1 and KhES3.	Cells were immunostained with anti-insulin antibodies and the Celigo was used to determine the induction rate of INS+ cells.
Huang, Ying	May 2017	Identification of hMex-3A and its effect on human bladder cancer cell proliferation	Oncotarget	Bladder cancer cells lines:5637 and T24	The Celigo was used to examine the proliferation after bladder cancer cells were transfected with an interference RNA sequence.
Grav, Lise	May 2017	Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells	Springer Link	CHO Cells	The Celigo was used to determine the cell survival and cell confluency.
Ivanov, Delyan	May 2017	High-Throughput Spheroid Screens Using Volume, Resazurin Reduction, and Acid Phosphatase Activity	Springer Link		References the Celigo as being capable of measuring spheroid size and volume in high throughput.
Chan, Leo	May 2017	A high-throughput image cytometry-based screening method for the detection of IL2-induced peripheral blood mononuclear cell-mediated cytotoxicity	Cytotherapy	peripheral blood mononuclear cells (PBMC)	"Viability was determined by employing different staining techniques such as enzymatic stain, nucleic acid stain, multi-stain method, fluorescent stain, and trypan blue staining on the Celigo and Cellometer. "
Chan, Leo	May 2017	Assessment of Cell Viability with Single-, Dual-, and Multi-Staining Methods Using Image Cytometry	Springer Link		In this work, they "demonstrate a novel high-throughput cytotoxicity screening assay using the Celigo imaging-cytometry method". A 96 well plate was used with the Celigo to analyze the percent lysis of cells.
Cuny, Gregory	April 2017	PYRIMIDINE COMPOUNDS AND METHODS USING THE SAME	United States Patent Application	SH-SY5Y-hTAU441 cell	The Celigo was used to assess the cytotoxicity of SH-SY5Y-hTAU441 cells.
Gheller, Brandon	April 2017	PYY regulates human skeletal muscle progenitor cell proliferation	The FASEB Journal	Skeletal Muscle stem cells (satelitte cells)	The Celigo was used to track percent confluence of cells over five days.
Blum, Jamie	April 2017	Short-term Inflammation Increases Proliferative Capacity of Human Skeletal Muscle Progenitor Cells from Young and Old Female Donors	The FASEB Journal	Muslce Progenitor Cells	The Celigo was used to track the percent confluence, number of nuclei, and percent cell death.
Smith, Paul	April 2017	Microenviroment Cytometry	Single Cell Analysis: Contemporary Research and Clinical Applications	multicellular tumor spheroids (MCTSs)	The Celigo was used to track the multicellular tumor spheroids health by staining with viability dye DRAQ7. They also looked at non-viable cells in the culture.
Zhang, Haohai	April 2017	Novel high-throughput cell-based hybridoma screening methodology using the Celigo Image Cytometer	Elsevier	Chinease hamster ovary (CHO) cells	"Demonstrate novel high-throughput screening methodology employing the Celigo Image Cytometer, which avoids nonspecific signals by contrasting antibody binding signals directly on living cells, with and without recombinant antigen expression."
Venugopal, Jessica	April 2017	Ouabain promotes partial epithelial to mesenchymal transition (EMT) changes in human autosomal dominant polycystic kidney disease (ADPKD) cells	Elsevier	kidney disease (ADPKD) cells	The Celigo was used in combination with an inverted microscope to monitor the wound healing of a cell monolayer.
Yang, Mei-Lin	March 2017	"IL-6 ameliorates acute lung injury in influenza virus infection"	Scientific Reports	fibroblasts isolated from IL-6 -/- mice and WT mice	Fibroblasts were isolated from IL-6-/- mice and WT mice. The Celigo was used to determine the cell count, doubling time, and apoptosis rate by immunofluorescence with anti-cleaved caspase-3 antibody. It was also used to monitor the number of virus infected cells engulfed by macrophages compared to IL-6/- macrophages. The macrophages were exposed to QD649 particles and stained with DAPI.
Itoh, M	March 2017	NanoCulture Plate: A scaffold‐based high‐throughput three‐dimensional cell culture system suitable for live imaging and co‐culture	Technology Platforms for 3D Cell Culture: A User's Guide		States how NCP can be used with the Celigo for measurments.
Slawny, Nicole	March 2017	Physiologically relevant spheroid models for three‐dimensional cell culture	Technology Platforms for 3D Cell Culture: A User's Guide		Lists the Celigo as a type of automated 3D assay equipment
Quentin Li, Qingdi	March 2017	Proteomic analysis of proteome and histone post-translational modifications in heat shock protein 90 inhibition-mediated bladder cancer therapeutics	Scientific Reports	5637 bladder cancer cells	They wanted to determine the antitumor impact of HSP90 inhibitors in 5637 bladder cancer cells. They stained the cells and used the Celigo to examine the cell viability.
Dall'Acqua, William	January 2017	Enhanced tumor-targeting selectivity by modulating bispecific antibody binding affinity and format valence	Scientific Reports	NCI-H358 non-small cancer cells	Cell cytotoxicity studies were performed on a Celigo S Imaging cytometer. Overlay of brightfield with either green fluorescence or blue fluorescence identified and qualified CMFDA or BMQC positive cells using Celigo Image processing software.
Li, Linfeng	January 2017	3D High-Content Screening of Organoids for Drug Discovery	Elsevier	None	This paper listed the Celigo as a 3D high content screening system
Parker, Christopher	January 2017	Ligand and Target Discovery byFragment-Based Screening in Human Cells	Elsevier	Human Cells
Cribbes, Scott	January 2017	A Novel Multiparametric Drug-Scoring Method for High-Throughput Screening of 3D Multicellular Tumor Spheroids Using the Celigo Image Cytometer	SAGE Journals	glioblastoma cell line U87MG	In this work, we employed the Celigo Image Cytometer to examine the effects of 14 cancer drug compounds on 3D MCTS of the glioblastoma cell line U87MG in 384-well plates.
Auberon, Florence	December 2016	Isolation of novel stilbenoids from the roots of Cyrtopodium paniculatum (Orchidaceae)	Fitoterapia	human glioblastoma U-87MG cells	Celigo
Braganza, Andrea	December 2016	UBE3B is a calmodulin-regulated, mitochondria-associated E3 ubiquitin ligase	The Journal of Biological Chemistry	human cells	The cells were counted various times after plating. Colonies were also stained with Crystal violet dye and counted with the Celigo.
Boss, Olivier	December 2016	Methods and compositions for inducing differentiation of human brown adiopocyte progenitors	FPO	adipose tissue (BAT) progenitor cells	Cells were exposed to C1-BODIPY® 500/510 C12 (Molecular Probes #D-3823) and incubated for 3 to 6 hours. C1-BODIPY® 500/510 C12 is a fluorescent fatty acid analog that is sometimes utilized for lipid trafficking studies (ThermoFisher). Next, the cells were analyzed in bright field and fluorescence. They looked at the total fluorescent intensity per well.
Wu, Yao	November 2016	Cucurbitacin-I induces hypertrophy in H9c2 cardiomyoblasts through activation of autophagy via MEK/ERK1/2 signaling pathway	Toxicology Letters	H9c2 cells	The cell viabilities were detected by the Celigo Image Cytometer
Kramer, Daniela	November 2016	Strong antitumor synergy between DNA crosslinking and HSP90 inhibition causes massive premitotic DNA fragmentation in ovarian cancer cells	Cell Death & Differentiation	human ovarian cancer cell lines
Su, X	November 2016	TAp63 suppresses mammary tumorigenesis through regulation of the Hippo pathway	Oncogene	mammary epithelial cells (MECs), mammary stem cells (MaSCs) and tumor-initiating cells (TICs)
Wu, Yao	November 2016	Cucurbitacin-I induceshypertrophy in H9c2 cardiomyoblasts through activation of autophagy viaMEK/ERK1/2 signaling pathway	Toxicology Letters	H9c2 cells	The Celigo was used to analyze cell viability of H9c2 cells
Cisneros Castillo, Liliana	October 2016	A novel computer-assisted approach to evaluate multicellular tumor spheroid invasion assay	Scientific Reports	tumor and peripheral cells	To quantify the size of MCTS in invasion assays, the present of the Celigo Cytometer is an approach that comes already with a device that takes images automatically of an inserted plate.
Kari, Vijayalakshmi	September 2016	Loss of CHD1 causes DNA repair defects and enhances prostate cancer therapeutic responsiveness	The Embo Journal	Prostate cancer cells
Hamilton, Duane	September 2016	Brachyury, a vaccine target, is overexpressed in triple- negative breast cancer	Society for Endocrinology	Triple-negative breast cancer cells	Nuclei were stained using DAPI and images were acquired using the Celigo S cell Imaging Cytometer
Potapova, Tamara	August 2016	Transcriptome analysis of tetraploid cells identifies cyclin D2 as a facilitator of adaptation to genome doubling in the presence of p53	Molecular Biology of the Cell	Tetraplid cells	Multi-well plates with labeled cells were imaged on Celigo Imaging Cytometer. Images were quantified and each image typically contained several hundred of cells; 3-4 images were analyzed to determine the mean number of positive cells and the standard deviation
Imamura, Yukio	August 2016	Near-Infrared Emitting Pbs Quantum Dots for in Vivo Fluorescence imaging of the Thrombotic state in septic mouse brain	Molecules	HeLa cells	HeLa cells were plated at 6000 cells/well in 96 well plates. After culturing overnight, QD1100 was added to every well at a final concentration of 0-50 nM. The number of cells in each well was counted with the Celgio after 0,7,24,4,8, and 60 hours.
Maguire, Sarah	August 2016	3D modelling identifies novel genetic dependencies associated with breast cancer progression in the isogenic MCF10 model	The Journal of Pathology	MCF10A cells	Comparison of pathway aberrations associated with progression identified that when cells are grown as 3D spheroids, they show perturbations of cancer-relevant pathways. The spheroids are was calculated using the Celigo S.
Chan, Leo Li-Yang	August 2016	A high-throughput AO/PI- based cell concentration and viability detection method using the Celigo image cytometry	Cytotechnology	Jurkat cells	The high-throughput AO/PI method described here allows for 96 well to 384-well plate samples to be analyzed in less than 7 mins, which greatly reduces the time required for the single sample based automated cell counter.
Shan, Feng	July 2016	Investigation of cancer drug penetration in 2D and 3D tumor cell culture models	University of Pittsburgh	Cal33 HNSCC cells
Bonasu, Surekha	July 2016	Real-time Caspase 3/7 measurement of suspension and adherent cells using the Celigo Image cytometer	Poster	Jurkat cells	Monitoring by detecting a green caspase 3/7 signal but also perform an end point assay by counterstaining the cells with Hoechst and therby determined a percent nucleated cells that are apoptosis by imaging on the Nexcelom Celigo image cytometer.
Chan, Leo Li-Yang	July 2016	A high-throughput image cytometry-based screening method for the cytotoxic effect of drug compounds on 3D tumor spheroid	Cancer Research	Cancer cells	Five experiments were conducted demonstrating comparable results in 2D and 3D models using the image based high-throughput screening method for 3D tumor spheroids on 384- well ultra low attachment round bottom microplates using the Celigo image cytometer.
Shirihai, Orian	July 2016	Lysosome acidification by photoactivated nanoparticles restores autophagy under lipotoxicity	The Journal of Cell Biology	B- cells	Analysis parameters for images acquired by the Celigo were optimized to identify individual cells based on fluorescence. Cells were then fixed in 4% paraformaldehyde, stained with DAPI, and imaged using Celigo to count the total number of cells per well for normalization.
Chan, Leo Li-Ying	June 2016	High-throughput 3D tumor spheroid screening method for cancer drug discovery using Celigo Image Cytometry	Journal of Laboratory Automation	U87MG	Celigo was used to determine the seeding density for the tumor sphere formation of the cell line U87MG, and it was to measure the IC50 value, and the dose responses of 17-AGG, paclitaxel, TMZ and doxorubicin
Ellegaard, Anne-Marie	June 2016	Repurposing Cationic amphiphilic antihistamines for cancer treatment	EbioMedicine	Non-small cell lung cancer (NSCLC)	Celigo measured the cell death after 15 minutes of propidium iodide and Hoechst-33342 staining was employeed at 37 degrees celcius.
Mazor, Yariv	June 2016	Enhancement of Immune Effector Functions by modulating IgG's intrinsic affinity for target antigen	Immune cells, T-cells	Immune cells, T-cells	Target cells were stained with Calcein AM dye, and then seeded in a 96 well plate at a density of 1x10^4 cells/well in RPMI in 1640 with GlutaMAX with 5% FBS. Calcein AM positive cells were quantified by Celigo.
Napoli, Marco	June 2016	ΔNp63/DGCR8- Dependent MicroRNAs mediate therapeutic efficacy of HDAC inhibitors in Cancer	Cancer cell	Squamous cell carcinomas and lymphoma cells	To acess the efficacy of the compounds in affecting the protein levels of ΔNp63 and DGCR8, evaluating cells by immunofluoresence and quantified them with Celigo.
Patterson, Lisa	May 2016	In vitro assays for endothelial cell functions required for angiogenesis: Proliferation, motility, tubular differentiation, and matrix proteolysis	Angiogenesis Protocols	Umbilical vein endothelial cells, microvascular endthothelial cells	Lysosomal areas in the indicated fibroblasts treated for 12 hours with vehicle (V) or 19.5 or 39um C12-SM were measured with Celigo adherent cell cytometer after staining.
Kildegaard, Helene Faustrup	May 2016	Accelerated homology-directed targeted integration of transgenes in Chinese hamster ovary cells via CRISPR/Cas9 and fluorescent enrichment	Biotechnology & Bioengineering	CHO cells	Lysosomal areas in the indicated fibroblasts treated for 12 hours with vehicle (V) or 19.5 or 39um C12-SM were measured with Celigo adherent cell cytometer after staining.
Tomokazu, Tanaka	April 2016	Low-dose farnesyltransferase inhibitor suppresses HIF-1a and snail expression in Triple-negative breast cancer MDA-MB-231 cells in vitro	Journal of Cellular Physiology	MDA-MB-TNBC and MDA-MB-231 cells	Tumorspheres were directly counted by the Celigo Cell Cytometer at a 12-day culture. Pictures of each well were taken to conform spheres and aggregates of cells.
Mattson, Emma	April 2016	Understanding the role of RB-binding protein KDM5A in cancer cell proliferation	Illinois Mathematics and Science Academy	lung cancer cells	Focused on clarifying the role of KDM5A in a non-small cell lung cancer line under different concentrations of erlotinib, a common chemotherapy drug, by examinging the cell cycle and cell proliferation using the Celigo.
Elisabeth, Corecelle-Termeau	April 2016	Excess sphingomyelin disturbsATG9A trafficking and autophagosome closure	Autophagy	SMPD-1- deficient cells	Lysosomal areas in the indicated fibroblasts treated for 12 hours with vehicle (V) or 19.5 or 39um C12-SM were measured with Celigo adherent cell cytometer after staining.
Amy, Wagers	April 2016	Methods and compositions forrejuvenating skeletal muscle stem cells	FPO	Skeletal muscle stem cells	Wells containing myogenic colonies were either conunted by brightfield microscopy or fixed with 4% PFA and the number of cells per well was counted on a Celigo automated microscope as Hoechst-stained nuclei at the indicated time points
Gayathri, Devi	April 2016	Three-Dimensional culturesystems in cancer research: Focus on tumor spheroid model	Pharmacology & Therapeutics	Cancer stem cells	Cancer cells propagated in three-dimensional (3D) culture systems exhibit physiologically relevant cell-cell and cell-matrix interactions, gene expression and signaling pathway profiles, heterogeneity, and structural complexity that reflecdt in vivo tumors.
Bidisha, Bhattacharya	April 2016	Fine-tuning of macrophageactivation using synthetic rocaglate derivates	Scientific Reports	J7-21 cells	Celigo cytometer was used ot measure number of GFP-Ipr1 positive cells and GFP-Ipr1 fluorescence intensity per nucleus after counterstaining with nuclear stain DAPI.
Peitzsch, Claudia	March 2016	An epigenetic reprogramming strategy to re-sensitize radioresistant prostate cancer cells	Cancer Research	Cancer stem cells	After 14 days, cells were anaylzed and automatically scanned using the Celigo for 30 mins at 37 degrees celcius, washed with PBS at the end of the culture, then analyzed by the Celigo.
Stanford, Elizabeth	March 2016	The role of the aryl hydrocarbonreceptor in the development of the cells with the molecular and functionalcharacteristics of cancer stem- like cells	BMC Biology	Breast cancer stem cells	After cells were treated, harvested, dosed, and plated colonies were quantified with the Celigo after eight days.
Mohammad, Tariq	January 2016	Eukaryotic translationinitiation factor 5A (elF5A) is essential for HIF-1a activiation in hypoxia	Biochemical and Biophysical research communications	Tumor spheroids	Suppression of elF5A by siRNA oligomediated knockdown or treamtent with GC7, a deoxyhypusine synthase inhibitor, led to significant reduction of HIF-1a activity
Edwin, Chang	November 2015	AshwaMAX and withaferin Ainhbits gliomas in cellular and murine orthotopic models	Journal of Neuro-oncology	GBM2, GBM39	Human parietal-cortical gliobastoma cells (GBM2, GBM39) were isolated from primary tumours while U87-MG was obtained commerically.
Linfeng, Li	November 2015	High-throughput imaging:Focusing in on drug discovery in 3D	Methods	MCT cells	3D organotypic culture models such as organoids and multicellular tumor spheroids (MCTS) are becoming more widely used for drug discovery
Henning, Gram Hensen	December 2015	Versatile microscale screeningplatform for improving recombinant protein productivity in chinese hamsterovary cells	Scientific Reports	Ovarian cells	The platform consists of four techniques compatible with 96-well microplates:lipid-based transient transfection, cell cultivation in microplates, cell counting and antibody-independent product titer determination based on split-GFP complementation
Sivapriya, Ponnurangam	December 2015	Quinomycin A targets notchsignaling pathway in pancreatic cancer stem cells	Oncotarget	CSC cells	Quinomycin treatment resulted in significant inhibition of proliferation and colony formation in pancreatic cancer cell lines, but not in normal pancreatic epithelial cells
Annika, Baude	December 2015	Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination	Nucleic Acids research	HeLa cervic carcinoma cells	POGZ-depleted cells show a similar defect in DNA damage-induced RPA2 phosphorylation as HDGFRP2-depleted cells
Andrew, Campbell	October 2015	Mutation ofataxia-telangiectasia mutated is associated with dysfunctional glutathionehomeostasis in cerebellar astroglia	Glia	Mouse cells	Cerebellar atroglia isolated from Atm mutant mice show decreased expression of the cystine/glutamate exchanger subunit xCT, glutathione (GSH) reductase, and glutathione-S-transferase.
Thilo, Riedl	October 2015	High-throughput screening forinternalizing antibodies by homogeneous fluorescence imaging of apH-activated probe	Journal of Biomolecular Screening	A431, AU565, SKOV-3 cells	The pH-activated CypHer5E label becomes fluorescent upon internalization into the acidic environment of endocytic organelles, whereas background fluorescence of noninternalized CypHer5E is minimal.
Narendran, Aru	September 2015	Targeted inhibition of MEK1 bycobimetinib leads to differentiation and apoptosis in neuroblastoma cells	BioMed central	None
Simeonov, Anton	September 2015	High- throughput fluorescence imaging approaches for drug discovery using in vitro and in vivo three-dimensional models	Expert Opinion Drug Discovery	None
Yoshida, Minoru	September 2015	Identification of thedeterminants of 2-deoxyglucose sensitivity in cancer cells by shRNA libraryscreening	ScienceDirect	Cancer cells
Kaji, Keisuke	September 2015	Reprogramming roadblocks are system dependent	ISSCR	(iPSC's)
Anant, Shrikant	August 2015	RNA binding protein RBM3increases B-catenin signaling to increase stem cell characteristics incolorectal cancer cells	Wiley Online Library	Cancer stem cells
McGlennen, R	July 2015	Comparison of Antimicrobial andwound healing agents on oral fibroblast viability and In-vivo bacterial load	Omics Online	Gingival fibroblast cells
Dobbelstein, Matthias	July 2015	MicroRNA-101 suppresses tumorcell proliferation by acting as an endogenous proteasome inhibitor viatargeting the proteasome assembly factor POMP	ScienceDirect	Tumor cells
Dubrovska, Anna	June 2015	Development of novelradiochemotherapy approaches targeting prostate tumor progenitor cells usingnanohybrids	International Journal of Cancer	Cancer stem cells
Gahl, William	June 2015	Automated, High-throughputderivation, characterization and differentiation of induced pluripotent stemcells	Nature Methods	(iPSC's)
Mermod, Nicolas	June 2015	Epigenetic regulatory elements:Recent advances in understanding their mode of action and use for recombinantprotein production in mammalian cells	Biotechnology Journal	mammalian cells
Lee, Dan	May 2015	High- throughput direct cell counting- based natural killer cell mediated- cytotoxicity assay using Celigo Imaging Cytometry	The Journal of Immunology	NK cells
Sun, Yi	May 2015	A reliable parameter tostandardize the scoring of stem cell spheres	PLOS one	Mouse embryonic stem cells
Jaattela, Marja	May 2015	Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay	Autophagy
Chang, Stephen	May 2015	Rapid and efficient generationof transgene-free iPSC from a small volume of cryopreserved blood	Stem cell reviews and reports	hESC	online
Kildegaard, Helene	April 2015	One-step generation of tripleknockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment	Biotechnology Journal	CHO	Online
Leach, M.O	March 2015	Acquired resistance to EGFRtyrosine kinase inhibitors alters the metabolism of human head and necksquamous carcinoma cells and xenograft tumors	British Journal of Cancer	CAL/PJ HNSCC	online
Nabergoj, Dominik, Elsa	December 2014	Evaluation of anti-inflammatory and proapoptotic activities of synthetic clathrodin analogues in human THP-1 monocytic Leukemia cells	Thesis	THP-1 cells	online
Flores, Elsa	November 2014	Non-disruptively count and quantify fluorescence iPS colonies during 2 degree reprogramming: 7 min per 6 well plate, dual- fluorescence whole well imaging cytometry	Nature	Mouse embryonic stem cells(G4)	online
Kolev, Vihren	November 2014	P13K/mTOR dual inhibitor VS-5584preferentially target cancer stem cells	American Association for Cancer Research	Cancer stem cells	To detemine tumorsphere forming efficency, cells from the tissue culture were plated and enumerated using the Celigo.
Karlin, Mondal	November 2014	The oncogenic STP axis promotestriple-negative breast cancer via degradation of the REST tumor suppressor	Cell Reports	BT549 and MDA-MB231-LM2 breast cancer cells	Celigo was used to perform cell proliferation assays.
Westbrook, Thomas	November 2014	The oncogenic STP axis promotestriple-negative breast cancer via degradation of the REST tumor suppressor	Cell Reports	None	online
Miura, Hiromi	October 2014	Development of spheroid based high-throughput screening of cell-cell adhesion inhibitors to reverse acquired multicellular resistance	Cancer Research	A549	online
Randhawa, Zeng	October 2014	Inhibition of large T antigenATPase activity as a potential strategy to develop anti-polyomavirus JC drugs	Antiviral Research	HEK293, COS7	Cell counts were evaluated by Celigo to ascertain cytotoxicity of compounds.
Glicksman, Marcie A.	October 2014	Inhibition of large T antigenATPase activity as a potential strategy to develop anti-polyomavirus JC drugs	Antiviral Research	HEK293, COS7	online
Landmann, Proia	September 2014	UDP glucuraonosyltransferase 1Aexpression levels determine the response of colorectal cancer cells to theheat shock protein 90 inhibitor ganetespib	Cell Death and Disease	human colorectal cancer cell lines	Cell confluence in drug treated and untreated wells was determined by Celigo with bright field.
Fu, Creighton	September 2014	Overcoming endocrine resistancedue to reduced PTEN levels in estrogen receptor-positive breast cancer byco-targeting mammalian target of rapamycin, protein kinase B, ormitogen-activated protein kinase kinase	Breast Cancer Research	MCF-7L, BT483, and T47D (human breast cancer)	Cell count was assessed on Celigo via methylene blue and bright field.
Schiff, R	September 2014	Overcoming endocrine resistancedue to reduced PTEN levels in estrogen receptor-positive breast cancer byco-targeting mammalian target of rapamycin, protein kinase B, ormitogen-activated protein kinase kinase	Breast Cancer Research	MCF7L (Human Breast Cancer), BT483, T47D	Online
Xu, Farach-Carson	July 2014	Three-dimensional in vitro tumormodels for cancer research and drug evaluation	Biotechnology Advances	tissue engineered-3D tumor models	Celigo measured spheroid diameter, permieter, and area which enabled assessments of motility, angiogenesis, and matrix invasion, as well as the actions of cell growth inhibitors.
Brix, Rafn	July 2014	Screening and identification ofsmall molecule inhibitors of ErbB2-induced invasion	Molecular Oncology	MCF-7 (human breast cancer) and SK-OV3 (human ovarian)	Cells were stained with Hoechst and PI and Celigo ascertained the total cell counts as well as number of dead cells.
Jayanthan, Ruan	July 2014	Aurora kinases as druggabletargets in pediatric leukemia: heterogeneity in target modulation activitiesand cytotoxicity by diverse novel therapeutic agents	PLOS One	pediatric and infant leukemia cell lines	Cell lines were stained with Alamar blue to assess cells survival via metabolic activity.
Scheerlinck, Van Steendam	June 2014	Detailed method description fornoninvasive monitoring of differentiation status of human embryonic stemcells	Analytical Biochemistry	human embryonic stem cells (hESCs)	Celigo evaluated colony confluence and measurement of stress level via Oct 4 expression.
Pederson, Ronda	May 2014	Accelerating genome editing inCHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool	Biotechnology and Bioengineering	CHO cells	Two-channel bright field and GFP images were captured by Celigo to count cells and determine transfection efficiency.
Lund, Kildegaard	May 2014	A versatile system for USERcloning-based assembly of expression vectors for mammalian cell engineering	PLOS One	CHO-S (Chinese hamster ovary clonal isolate)	Cells were plated in 6-well plates and transfected with GFP-tagged vectors before the addition of Hoechst stain. Two-channel (Hoechst and GFP) images were collected on the Celigo to determine cell count and transfection efficiency.
Guo, Loh	May 2014	Development of a magnetic 3Dspheroid platform with potential application for high-throughput drugscreening	Molecular Pharmaceutics	MCF-7 (human breast cancer)	Bright field images of the spheroids in 96-well plates were generated over a time course by Celigo. Growth kinetics of spheroid diameter, perimeter, and area were captured.
Jaiswal, Lauritzen	May 2014	S100A11 is required forefficient plasma membrane repair and survival of invasive cancer cells	Nature Communications	MCF-7 (human breast cancer)	Cell death was determined by Celigo using propidium idodide (PI) 96 hours after control or siRNA delivery.
Yang, Chung	May 2014	Systems analysis of the prostatetumor suppressor NKX3.1 supports roles in DNA repiar and luminal celldifferentiation	F1000 Research	LNCaP (human prostate cancer)	Cells were dual stained with Heochst and Alexa Fluor 594 and imaged by Celigo to ascertain cell proliferation.
Guo, Loh	May 2014	Development of a magnetic 3Dspheroid platform with potential application for high-throughput drugscreening	Molecular Pharmaceutics	multicellular tumor spheroids	Celigo captured bright field images over time to determine diameter, perimeter, and area of the spheroids with or without drug treatment.
Kalineo, Thein	April 2014	Acetaminophen and NAPQI aretoxic to auditory cells via oxidative and endoplasmic reticulumstress-dependent pathways	Hearing Research	HEI-OC1 cells (murine-derived auditory cells)	Celigo performed direct cell counts after treatment with control or drug over a time course.
Spike, Kelber	April 2014	CRIPTO/GRP78 signaling maintainsfetal and adult mammary stem cells ex vivo	Stem Cell Reports	MCF10A and fetal mammary stem cells	Celigo measured DNA content via Hoechst and cell proliferation through bright field images.
Vissapragada, Contreras	March 2014	Bidirectional crosstalk betweenperiventricular endothelial cells and neural progenitor cells promotes theformation of a neurovascular unit	Brain Research	Periventricular region endothelial cells (PVEC) and neural progenitor cells (NPC)	Cells were imaged by Celigo in 96-well Matrigel coated plates to ascertain the length and pixel intensity of 3D cords created by GFP-labeled ECs.
Holembowski, Kramer	March 2014	TAp73 is essential for germ celladhesion and maturation in testis	Journal of Cell Biology	Germ-Stertoli cell co-cultures	Celigo captured two-channel images (bright field and GFP) to quantify cells after lentiviral infection of adhesion genes.
Emhemmed, Azouaou	March 2014	Selective anticancer effects ofa synthetic flavagline on human Oct4-expressing cancer stem-like cells via ap38 MAPK-dependent caspase-3-dependent pathway	Biochemical Pharmacology	NT2/D1 (human embryonal carcinoma)	Celigo determined the rate of apoptosis via Annexin V/PI staining after flavagline derivative FL3 application.
Trapnell, Cacchiarelli	March 2014	The dynamics and regulators ofcell fate decisions are revealed by pseudotemporal ordering of single cells	Nature Biotechnology	human skeletal musucle myoblasts	Celigo performed whole well imaging with Hoechst and markers to determine the number of cells or nuclei, fraction of nueclei in MYH2- or CD13-positive cells, and total MYH2-positive area.
Shih, Chung-Hsuan	February 2014	Astroglial-Derived PeriostinPromotes Axonal Regeneration after Spinal Cord Injury	The Journal of Neuroscience	Rat Neurites	Neurite length was measured using Celigo cytometer to quantify the confluence of TujI-stained neurites, providing a quantitative measurement of neurite outgrowth.
Fu, Morrison	February 2014	Therapeutic potential of thedual EGFR/HER2 inhibitor AZD8931 in circumventing endocrine resistance	Breast Cancer Research Treatment	Tamoxifen resistant (TamRes) MCF-7 and T47D cells (both breast cancer)	Cells counts and rate of apoptosis via Annexin V-FITC staining was determined by Celigo.
Schulz, Ramona	January 2014	HER2/ErbB2 Activates HSF1 andThereby Controls HSP90 Clients Including MIF in HER2-Overexpressing BreastCancer	Cell Death and Disease	MDA-MB-453, and SK-BR-3 (Human Breast Cancer)	Cells were seeded and cultured for 1 day, then treated with CP724.714 (CP) for 24hr or left untreated and followed up to day 5. Cell confluence measured daily by Celigo. For cell survival, equal numbers of treated or untreated cells were plated into 12 well plates. With the Celigo cytometer, cell confluence was measured over indicated time periods w/Celigo
Sproul, Andrew	January 2014	Characterization and MolecularProfiling of PSEN1 Familial Alzheimers Disease iPSC Derived NeuralProgenitors	PLOS one	Fibroblast 11 and 11C, reprogramed to iPSCs	Immunostaining was performed. Hoechst was used to visualize DNA. The following antibodies were used: OCT4, SSea4, Nanog, Tra-160, Ki67, MAP2, Nestin, NeuNm TujI, NLRP2, NDP. Quantification of immunostaining was done on the Celigo 200-BFFL
Giovannini, Marco	January 2014	mTORC1 Inhibition Delays Growthof Neurofibromatosis Type 2 Schwannoma	Neuro-Oncology	Human Primary Schwann cells	Cells were fized in 4% paraformaldehyde and stained with anti-S100 protein,, followed by AI549-conjugated goat anti-rabbit secondary antibody and counterstained with Hoechst 33258. Cell surface areas were determined using the automated Celigo Cytometer.
Postupalenko, Viktoriia	January 2014	Intracellular Delivery ofFunctionally Active Proteins Using Self-AssemblingPyridulthiourea-polyehtlenimine	Journal of Controlled Release	U87 (Human Glioma), CaSki (Human small bowel mesentery), HeLa (Human Cervical Cancer)	Cells were seeded into 96-well plates at 10 4 cells/well the day before . Flow cytometry analysis was performed with a Celigo Cytometer after a 24 h incubation period on a suspension of freshly trypsinized cells
Postupalenko, Sibler	January 2014	Intracellular delivery offunctionally active proteins using self-assemblingpyridylthiourea-polyethylenimine	Journal of Controlled Release	U87 (human primary glioblastoma)	Cells were plated in 96-well plates and treated with GFP polyplexes. Celigo was used to determine intracellular eGFP delivery.
Schulz, R.	January 2014	HER2/ErbB2 Activates HSF1 andThereby Controls HSP90 Clients Including MIF in HER2-Overexpressing BreastCancer	Cell Death and Disease
Ferree, Andrew	2014	Supplemental Material-characterization and molecular profiling of PSEN1	Landes Bioscience
Venkatanarayan, Raulji	2014	IAPP-driven metabolicreprogramming induces regression of p53-deficient tumours in vivo	Nature	H1299 (human lung adenocarcinoma)	Rates of apoptosis (via Annexin V/PI staining) and ROS generation (via CellRox Deep Red) were determined by Celigo.
Chen, Hsing-Yu	March 2013	Inhibition of red/Fyn/c-CBLPathway Function by Cdc42 Controls Tumour Initiation Capacity and TamoxifenSensitivity in Basal-like Breast Cancer Cells	EMBO Molecular Medicine	MDA-MB-231, MDA-MB-468, Hs578T, HCC1569, MCF-7m HCC38, HCC70, HCC1954, MCF-10A (Human Breast Cancer)	Cells were incubated with Calcein AM and PI for 15 mins, after which live and dead cells were counted utilizing the Celigo Cytometer. Levels of intracellular stress of oxidation were determined by CMH2DCFDA staining to the manufacturers instructions. Briefly cells were incubated with CM-H2DCFDA in the phenol red free medium in teh dark for 30 min, after which cells were washed once and fluorescent intensity of cells was determined using Celigo.
Proschel, Christoph	December 2013	Delayed Transplantation ofPrecursor Cell-Derived Astrocytes Provides Multiple Benefits in a Rat Modelof Parkinsons	EMBO Molecular Medicine	Cortical and dopaminergi mid brain neurons isolated from E18.5 Sprague-Dawley rats.	Cell survival was determined by co-labelling cultures with TujI and anti-tyrosine hydroxulase antibody. TujI+ or TujI+/TH+ neurons were counted using a Celigo Cytometer.
Neradugomma, Naveen	December 2013	Prolactin Signalling EnhancesColon Cancer Stemness by Modulating Notch Singaling in a Jak2-STAT3/ERKManner	Carcinogenesis	Colon cancer cell LinesHT29, HCT116, SW480, SW620, DLD1, and normal intenstinal epithelial fetal human colon (FHC) cells	Colosphere formation was assessed after 4-6 days, the number and size of colonospheres was determined using Celigo
Xu, Cong	November 2013	A Zebrafish Embryo CultureSystem Define Factors that Promote Vertebrate Myogenesis Across Species	Cell	Zebrafish cells	Zebra fish cells were iamged after 2 days using a Celigo cytometer for GFP, mCherry and BR signals.
Neradugomma, N.	November 2013	Prolactin induces Notchsignaling in a Jak2-STAT and Jak2-ERK pathway in colon cancer stem andprogenitor cells	Carcinogenesis
Zangi, Lior	September 2013	Modified mRNA Directs the Fateof Heart Progenitor Cells and Induces Vascular Regeneration After MyocardialInfarction	Nature Biotechnology	Skeletal muscle myotubes differentiated from primary mouse satellite cells	Cells were harvested and equal numbers were plated in each well of a 96-well plate in growth media. Cells were transfected with modified RNA after 3 d in differentiation media. Myotubes were transfected w/0, 0.3, 1 or 3 µg of GFP-modified RNA. Cells were fixed 16 h after transfection. Transfected myotubes were stained for skeletal muscle myosin heavy chain, 10 µg/ml Hoechst and rabbit anti-GFP Alexa-488. Pictures were taken from the whole well using the Celigo cytometer under blue, red and green channels. Cell viability was measured by quantifying the total number of nuclei in the transfected wells 16 h after transfection and normalizing them to the total number of nuclei in the non-transfected wells.
Chen, Hsing-Yu	September 2013	MEK1/2 Inhibition SuppressesTomixfen Toxicity on CNS Glial Progenitor Cells	The Journal of Neuroscience	O-2A/OPC (Astrocyte progentior/oligodendrocyte precursor cells), GRP (Glial-restricted precursor cells), astrocytes, neuroepithelial stem cells and oligodendrocytes	Cell number and death of O-2A/OPCs were analyzed by Calcein AM and PI Staining in live culture using a Celigo Cytometer.
Ferree, Andrew	September 2013	MitoTimer Probe Reveals theImpact of Autophagy, Fusion and Motility on Subcellular Distribution of Youngand Old Mitochondrial Protein and on Relative Mitochondrial Protein Age	Autophagy	MEF (Human Breast Cancer), COS (Monkey Kidney Tissue)	To quanity red and green fluorescence intensity per cell in MEF and COS cells, cells were imaged using Celigo. Analysis parameters for images acquired by Celigo were optimized to identify individual MEF and COS cell based on fluorescence and the avg. green and red fluorescnce intensity for each cell was determined at the different time points.
Wang, Ying	August 2013	Scalable Expansion of HumanInduced Pluripotent Stem Cells in the Defined Xeno-fress E8 Medium UnderAdherent and Suspension Culture Conditions	Journal of Stem Cell Research	BC1 (human primary effusion lymphoma ), TNC1 (Rat type 1 astrocytes)	The Total # of cell aggregates in a 1ml sample was analyzed by using the embryoid body analysis module in Celigo Imaging Cytometer. The avg. equivalent diameter of aggregates and the szie of each aggregate in the sample were also measured.
He, Xianbao	August 2013	The sst1 Resistance LocusRegulates Evasion of Type 1 Interferon Signalling by Chlamydia pneumoniae asa Disease Tolerance Mechanism	PLOS one: Pathogens	Bone Marrow Derived Macrophages	T-cell survival Assay: Cells were plated and treated with infected C. pneumoniae and at designated time points were stained with Hoechst and PI. Total cell number and dead cell number were counted using Celigo Cytometer
Li, Jianqing	June 2013	A Short Hairpin RNA Screen ofInterferon-Stimulated Genes Identifies a Novel Negative Regulator of theCellular Antiviral Response	Mbio.	Vero T144 (Kidney Epithelial Cells), NIH 3T3 (Human Fibroblasts), Hek293T (Human Embryonic Kideny cells), HeLa (Human Cervical Cancer)	HeLa cells in 96-well plates were transfected with individual ISGs tagged with 3X Flag. After 24h, cells were infected w/WNV for another 24h and then ﬁxed, permeabilized, and costained for WNV envelope protein (MAbE18)and the nucleus using DAPI. Images were captured and processed using a Celigo cytometer (Cyntellect). Infected and uninfected populations were gated separately, and infectivity was measured as the % of infected cells from the total cell counts.
Huang, Haiqing	June 2013	iPSC-Derived B-Cells ModelDiabetes Due to Glucokinase Deficiency	Journal of Clincial Investigation	Patient Cell derived IPS to B-cell Model	Quantification of positively stained cells was performed using the celigo cytometer system.
Bian, Shu	April 2013	P2X7 Integrates PI2k/AKT andAMPK-PRAS40-mTOR Signaling Pathways to Mediate Tumor Cell Death	PLOS one	MC138 (Colon Cancer Cell Line), B16/F10 (Melanoma cell line)	Cells were seeded into Corning 3603 Black 96-well plates and grown for 24 hr before exposed to ATP for a short period of time. 16–24 hr later, cell growth was evaluated using the Celigo. BR images of live cells were captured using the Celigo Cell Counting application
Escribano-Diaz, Cristina	March 2013	A Cell Cycle Dependent RegulatoryCircuit Composed of 53BP1-RIF1 and BRCA1-CtlP Controls DNA Repair PathwayChoice	Molecular Cell	U2OS (human Osteosarcoma)	U2OS cells were transfected with siRNA in 96-well plates. At 48 hr post-transfection cells were treated w/neocarzinostatin for 15 min. After a 3 hr recovery, cells were extracted with 0.2% Triton X-100 in PBS for 10 min on ice, ﬁxed with 4% paraformaldehyde (PFA), and processed for RPA32 immoﬂuorescence. Nuclei were counterstained with DAPI. Cells were imaged using the Celigo laser scanning plate cytometer (Brooks Automation) and analyzed with the accompanying image analysis software.
Vinci, Maria	January 2013	Tumor Spheroid Based Migration Assays for Evaluation of Therapeutic Agents	Methods in Molecular Biology
Lesovaya, Ekaterina	January 2013	Combination of a SelectiveActivator of the Glucocorticoid Receptor Compound A with a Protease Inhibitoras a Novel Strategy for Chemotherapy of Hematologic Malignancies	Cell Cycle	CEM (T- cell acute lymphoblastoma leukemia), K562 ( chronic myeloblastic leukemia), NCEB (mantle cell lymphoma) and multiple MM.1R (glucocorticoid resistant) and MM1S (glucocorticoid sensitive)	The proliferation was measured by direct cell counting using Celigo Cytometer. Cells were plated at 10^4/well onto 24-well plates and cultured in complete medium in the presence of CdpA, Dex, Bortexomib or vehicle.
Signh, Anjali	January 2013	Profiliing Pathway SpecificNovel Therapeutics in Preclinical Assessment for CNS Atypical TeratoidRhabdoid Tumors	Molecular Oncology	BT12 and BT16 (CNS ATRT), KCCF1 (cerebral spinal fluid), Hs68 (Primary skin fibroblast), T98G [EGFR over-expressing glioblastoma multiform (GBM)]	ATRT cells were trypsinized and placed in 96-well plates @ a concentration of 5x10^3 cells per well. Increasing concentrations of study agents were added to these wells to a final volu,e of 200ul per well. After 4 days, cell survival was quantified by Celigo Cytometer
Al-kasspooles, Mazin	January 2013	Preclincal Antitumor Activity ofa Nanoparticulate SN38	Journal of Investigative New Drugs	HT29, HCT116 (Human Colorectal Carcinoma) and H-meso-1 H226, H2052, MSTO-211H (Human Mesothelioma)	Cells were plated in 96-well black clear bottom plates. After overnight incubation, PI was added and live and dead cell numbers at time 0 were determined using the Celiigo cytometer. Drug treatments were then added to cells . Live and dead cell numbers were analyzed again 24, 48, and 72 after drug addition.
Singh, Lun	January 2013	Profiling pathway-specific noveltherapeutics in preclinical assessment for central nervous system atypicalteratoid rhabdoid tumors (CNS ATRT): Favorable activity of targeting EGFR-ErbB2 signaling with lapatinib	Molecular Oncology	atypical teratoid rhabdoid tumor (ATRT) cells	Cell survival in 96-well plates was determined by Celigo in bright field after 4 days treatment with drug or control.
Stecklein,	August 2012	SI Methods	PNAS
Golipour, Azadeh	December 2012	A Late Transition in SomaticCell Reprogramming Requires Regulators Distinct From the Pluripotency Network	Cell: Stem Cell	MEFs	Secondary MEFs as indicated in the schematics were stained for AO and DAPI and then scanned with a Celigo hgih-content microscope to quantigy AP-positive areas. Dox withdrawals was quantified for 4 SC and 4 SI clones using the Celigo system. In both screens, 3 days after transfection, cells were fixed and stained for Dppa24, counterstained with DAPI and then subjected to automated analysis using the Celigo system for quantifying colony formation
Smith, Karen	September 2012	Human Family with SequenceSimilarity 60 Member A (FAM60A) Protein: a New Subunit of the Sin3Deacetylase Complex	Molecular Cell Proteomic	A549, HepG2 (Human Liver Cancer)	A549 or HepG2 cells were transfected with control siRNAs or siRNAs against FAM60A. 3 days after transfection, cells were incubated for 5 hrs (A549) or 20hrs (HepG2) with BrdU. Cells were costained with DAPI. % incorporation of BrdU in each well was measured by fluorescence analysis using a Celigo Ceytometer
Stecklein, Shane	August 2012	BRCA1 amd HSP90 Cooperate inHomologous and Non-Homologous DNA Double Strand Break Repair and G2/MCheckpoint Activation	PNAS	HCC1937 (Human Breast Cancer)	DNA Synthesis, apoptosis and cell cycle distribution experiments in HCC1937 cells were performed on an LSRII flow cytometer or a Celigo adherent cell cytometer.
Nabzdyk, Christoph	August 2012	Differential Susceptibility ofHuman Primary Aortic and Coronary Artery Vascular Cells to RNA Interference	Journal of Biochemical and Biophysical Research Communications	Endothelial (EC) and vascular smooth muscle (SMC) cells	Cells were seeded at a density of 5000 cells/well of a 96-well plate. 24 hrs later cells were transfected with either non-targeting siRNA or non-targeting fluorescent red labelled siRNA using no transfection reagent., HiPerfect or Lipfectamine RNAiMax. Hoechst nuclei stain was used to label cells for counting. For data analysis an adherent cell cytometer Celigo was used.
McKevitt, I.	April 2012	SARS ORAL 2012	British Journal Of Surgery Society Ltd.
Borrego-Diaz, Emma	April 2012	Overactivation of Ras SignalingPathway in CD133+ MPNST Cells	Journal of Neurooncology	MCF7L (Human Breast Cancer)	Functional analysis of cell growth and apoptosis was performed following knockdown of miRNAs using insitu cell cytometry (Celigo)
Healy, N.A.	April 2012	The Role of miRNAs in TamoxifenResistance in Breast Cancer	Cellular and Molecular Life Sciences	Human MPNST primary cells and mouse MPNSTs, S805, S462, T530, T532, SW10 (mouse Schwann cells) and HSCs	The spheres were counted after 40hr, 7 days and 10 days of incubation using both Celigo and a light microscope.
Hsieh, Jeng-Long	March 2012	Acquisition of an EnhancedAggressive Phenotypein Human Lung Cancer Cells Slected by Suboptimal Doses ofCisplatin Following Cell Deattachment and Reattachment	Journal of Cancer Letters	A549, H1299, H1299-RI~H1299-R5 (Human Lung Cancer Cell Lines)	To analyze cell proliferation 1000 cells were seeded in 96-well plates in the complete medium at 37C on day 0. The number of cells was counted daily from day 1 to day 4 using a Celigo cytometer according to themanufacturers instructions. The proliferation rate is expressed as a ratio of the numberof cells counted on days 2-4 by the number counted on day 1
Vinci, Maria	March 2012	Advances in Establishment andAnalysis of 3D Tumor Spheroid-based Functional Assays for Target Validationand Drug Evaluation	BMC Biology	U-87 MG glioblastoma, SF188 glioblastoma, MDA-MB-231 (Human Breast Carcinoma),…37 other cancer cells lines.	For rapid, routine imaging and analysis of tumor spheroids, we utilized a Celigo cytometer, which is a bench top in situ celluar analysis system providing high quality, ful or partial images of wells using BR or FL illumination.
Huang, Han-Hung	February 2012	A Replacement for IsletEquivalents with Improved Reliability and Validity	Acta Diabetologica	Murine Pancreatic Islet cells	Isolated islet cells were iamged and counted with Celigo Cytometer
Ponnurangam, Sivapriya	February 2012	Honokiol in Combination withRadiation Targets Notch Signalling to Inhibit Colon Cancer Stem Cells	Molecular Cancer Therapeutics	HCT-116 (Human Colon Carcinoma), SW480 (Human Adenocarcinoma of the colon)	Colonosphere assay: The number and size of colonosphere were determined using Celigo
Schulz, Ramona	January 2012	Inhibiting the HSP90 ChaparoneDestabilizes Macrophage Migration Inhibitory Factor and Thereby InhibitsBreast Tumor Progression	Journal of Experimental Medicine	U2SO (Osteosarcoma), SW480 (Human adenocarcinoma of the colon)	Cell Confluence and cell numbers were evaluted over time by the Celigo Cytometer. Cell numbers (U2OS) or cell confluence (SW480) were measured using the Celigo cytometer using 49 squares per well
Gall, Jonathan	January 2012	Role of Mitofusin 2 in the RenalStress Response	PLOS one	Primary Murine Kidney Cells	Cells were fixed in 4% paraformaldehyde for 10 min, washed with PBS and stained with PBS containing Hoechst for 30 min before being imaged and counted by Celigo cytometer.
Li, Dan	January 2012	The Role of CYP3A4 mRNATranscript with Shortened 3'-Untranslated Region in HepatocyteDifferentiation, Liver Development and Repsonse to Drug Induction	Molecular Pharmacology	HepaRG Cells (Human Hepatic Cells)	The expression of RFP was analyzed in situ on an adherent cell cytometer to obtain cells images in both BR and red fuorescence, The ratios of cell densities from BR to red fluorescence in each entire well were analyzed by Celigo software.
Yoshida, Shunsuke	November 2011	Thrombospondin-2 Gene Silencingin Human Aortic Smooth Muscle Cells Improves Cell Attachment	Journal of the American College of Surgery	HAoSMC (Human Aortic Smooth Muscle Cell)	Scratch assay (wound healing attchment assay): cell positions were determined using BR and Confluence modes of Celigo, and cell counting of cells stained with Hoechst was performed
Majumdar, Ishita	November 2011	Synthetic Cyclohexenyl ChalconeNatural Products Possess Cytotoxic ActivitiesAgainst Prostate Cancer Cellsand Inhibit Cysteine Cathepsins in Vitro	Journal of Biochemical and Biophysical Research Communications	DU-145 and PC3 (Human Colon Cancer) Cells	Apoptotic cells stained with Hoechst were quantitatively analyzed with the Celigo cyotmeter
Wang, Yen-Chao	November 2011	Different Mechanisms forResistance to Trastuzumab Versus Lapatinib in HER2- Positive Breast Cancers,Role of Estrogen Receptor and HER2 Reactivation	Breast Cancer Research	BY474, UACC-812, AU-565, HCC-1569, HCC-1954, HCC-202, MDA-MB-361, MDA-MB-453, ZR75-30, SKBR-3, MCF7-HER2 (All Human Breast Cancer	Cells were incubated with Annexin V-FITC and DAPI for 30 min and analyzed by the Celigo Cytometer for Apoptosis. Cells were stained with EdU and the proliferation rate was analyzed by the Celigo cytometer
Hoeksema, Kimberly	September 2011	Systematic in-Vitro Evaluationof the NCI/NIH Developmental Therapeutics Program Approved Oncology Drug Setfor the Identification of a Candidate Drug Repertoire for MLL-RearrangedLeukemia	OncoTargets and Therapy	KOPN8 (B-Cell Precursor Leukemia), SEM (Human Acute Lymphoblastic Leukemia), B1 (Human B-cells), MOLT-3 (Human T-Lymphoblast), TIB-202 (acute monocytic leukemia) and Patient Bone Marrow Stromal (BMS) cells	Bright Field cell counting, viable cell numbers count and counting of primary leikemia samples.
Las, Guy	August 2011	Fatty Acids Suppress AutophagicTurnover in B-Cells	Journal of Biological Chemistry	INS1832/13 (Insulin-Producing ß-Cell Line)	Images of cells stained with Hoescht and PI, in paper.
Wlodkowic, Donald	May 2011	Wormomtry-on-a-Chip: InnovativeTechnologies for in Situ Analysis of Small Multicellular Organisms	Cytometry Part A.	None	Mentioned as an example of an image cytometer
Lesovaya, E.A.	May 2011	Antitumor Effect of Non-stroidGlucocorticoid Receptor Ligand CpdA on Leukemia Cell Lines CEM and K562	Biochemistry (Moscow)	K562 (human myelogenous leukemia) cells, CEM (human lymphoblastic leukemia), transfected to create CEM-NF-_B-GFP-Luc, K562-NF-_B- GFP-Luc, CEM-AP1-GFP-Luc, and K562-AP1-GFP-Luc	Direct Count of suspension cells and a good cell count curve as an in paper figure
Nabzdyk, Christoph	April 2011	High-Throughput RNAi AssayOptimization Using Adherent Cell Cytometry	Journal of Translational Medicine	Human Aortic Smooth Muscle Cells	Cells were stained with Cell Tracker Green (Invitrogen) and with Hoechst nuclei stain. Plates were read using the adherent cell cytometer equipped with a brightfield and three fluorescent channels: a blue filter for the Hoechst, red filter for the siGLO Red, and green for the Cell Tracker Green cytoplasmic dye.
Tingen, Candace	March 2011	A Macrophage and ThecaCell-Enriched Stromal Cell Population Influences Growth and Survival ofImmature Murine Follicles in Vitro	Society for Reproduction and Fertility	Live, adhered ovarian stroma cells (Mouse)	Imaging and cell counts taken from cells stained with Hoechst and FITC
Feng, Lili	March 2011	Vascular CD39/ENTPD1 DirectlyPromotes Tumor Cell Growth by Scavenging Extracellular AdenosineTriphosphate	Neoplasia	U251 (Human Neuronal Glioblastoma), MDA-MB-231 (Human Breast Adenocarcinoma), A431 (Human Epidermal Carcinoma) and 5637 (Human Bladder Carcinoma)	Cell conﬂuence was determined regularly for 16 days using the Celigo Cell Cytometer
Bug, M	March 2011	Anthracyclines induce theAcculumation of Mutant p53 Through E2F1-Dependant and Independent Mechanisms	Oncogene	Luciferase-expressing B16/F10, C57BL/6 (mouse melanoma) and Syngeneic C57BL/6murineMCA38 (colon cancer)	Cell growth of cells exposed to ATP was evaluated with images and cell counting using the Celigo counter. Images present in paper
Kaestner, Phillip	February 2011	Therapeutic Targeting of theMitotic Spindle Checkpoint Through Nanoparticle-Mediated siRNA DeliveryInhibits Tumor Growth in Vivo	Journal of Cancer Letters	HCT116 Cells (Human Colon Carcinoma)	Read cell numbers at 24hr increments
Wlodkowic, Donald	July 2010	Cytometry in Cell NecrobiologyRevisited. Recent Advances and New Vistas	Cytometry Part A.	None	Only mentioned as a comparable cytometric thenologu
Sirmaci, Asli	May 2010	A Truncating Mutation inSERPINB6 is Associated with Autosomal Recessive Nonsyndromic SensorineuralHearing Loss	The American Jounal of Human Genetics	HeLa cells, (Human Cervical Cancer) Transfected with SERPINB6-WT-GFP and SERPINB6-MUT-GFP	3-Channel, 8-bit stitched images were generated covering whole wells to identify the surface area & # of cells along with fluorescent intensities.
Hart, C.P.	May 2010	Product Focus: High-ContentScreening and Imaging: Instrumentation, Analysis and Applications	Journal of Biomolecular Screening	None	Product focus, describeds product capabilities