Measure the Cell Outgrowth from a Spheroid onto an Extra Cellular Matrix (ECM)

  1. Directly image tumor spheroids in various microwell formats
  2. Non-invasive bright field imaging allows the user to image the same plate over multiple days
  3. Perform a two-color fluorescent viability assay

Introduction

The Celigo imaging cytometer has been developed to fully automate imaging and analysis of 3D spheroids. This automated morphometric analysis tool significantly reduces the time and effort needed to quantify key aspects of 3D spheres including size, growth, growth tracking over time and response to chemotherapeutics.

Celigo imaging process

Measured Outgrowth from a HUVEC 3D Tumor Spheroid

Spheroid

Bright Field Spheroid image

Bright field image

Identified spheroid with cell outgrowth

Bright Field Identified Spheroid with cell outgrowth

Bright field image

Monitoring Cell Outgrowth from a GFP-labeled HUVEC Spheroid

Bright Field Identified Spheroid GFP

Spheroid

Bright Field Spheroid image

Bright field image

Spheroid fill view

Bright Field Identified Spheroid fill view image

Bright field image

 

3d tumor Spheroid outgrowth image image
3d tumor Spheroid outgrowth image image
3d tumor Spheroid outgrowth image image
3d tumor Spheroid outgrowth image image
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